Acquisition of Avian Pathogenic Escherichia coli Plasmids by a Commensal E. coli Isolate Enhances Its Abilities To Kill Chicken Embryos, Grow in Human Urine, and Colonize the Murine Kidney

dc.contributor.author Skyberg, Jerod
dc.contributor.author Nolan, Lisa
dc.contributor.author Johnson, Timothy
dc.contributor.author Johnson, James
dc.contributor.author Clabots, Connie
dc.contributor.author Logue, Catherine
dc.contributor.author Nolan, Lisa
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:27:00.000
dc.date.accessioned 2020-07-07T05:15:31Z
dc.date.available 2020-07-07T05:15:31Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.embargo 2013-05-03
dc.date.issued 2006-11-01
dc.description.abstract <p>We have found an avian pathogenic <em>Escherichia coli</em> (APEC) plasmid, pAPEC-O2-ColV, which contains many of the genes associated with APEC virulence and also shows similarity in content to a plasmid and pathogenicity island of human uropathogenic <em>E. coli</em> (UPEC). To test the possible role of this plasmid in virulence, it was transferred by conjugation along with a large R plasmid, pAPEC-O2-R, into a commensal avian <em>E. coli</em> strain. The transconjugant was compared to recipient strain NC, UPEC strain HE300, and donor strain APEC O2 using various assays, including lethality for chicken embryos, growth in human urine, and ability to cause urinary tract infection in mice. The transconjugant killed significantly more chicken embryos than did the recipient. In human urine, APEC O2 grew at a rate equivalent to that of UPEC strain HE300, and the transconjugant showed significantly increased growth compared to the recipient. The transconjugant also significantly outcompeted the recipient in colonization of the murine kidney. These findings suggest that APEC plasmids, such as pAPEC-O2-ColV, contribute to the pathogenesis of avian colibacillosis. Moreover, since avian <em>E. coli</em> and their plasmids may be transmitted to humans, evaluation of APEC plasmids as possible reservoirs of urovirulence genes for human UPEC may be warranted.</p>
dc.description.comments <p>This article is from <em>Infection and Immunity </em>74, no. 11 (November 2006): 6287–6292, doi:<a href="http://dx.doi.org/10.1128/IAI.00363-06" target="_blank">10.1128/IAI.00363-06</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/9/
dc.identifier.articleid 1006
dc.identifier.contextkey 4106723
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/9
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92399
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/9/2006_SkybergJA_AcquisitionAvianPathogenic.pdf|||Sat Jan 15 02:20:07 UTC 2022
dc.source.uri 10.1128/​IAI.00363-06
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Acquisition of Avian Pathogenic Escherichia coli Plasmids by a Commensal E. coli Isolate Enhances Its Abilities To Kill Chicken Embryos, Grow in Human Urine, and Colonize the Murine Kidney
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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