Successes and challenges in the developmemt of baculovirus-based vaccines for swine influenza and porcine circovirus type 2b

dc.contributor.advisor Cathy L. Miller
dc.contributor.advisor Eric M. Vaughn
dc.contributor.author Hernandez, Luis
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-08-11T15:47:36.000
dc.date.accessioned 2020-06-30T02:56:23Z
dc.date.available 2020-06-30T02:56:23Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.embargo 2015-10-14
dc.date.issued 2015-01-01
dc.description.abstract <p>Respiratory disease is a major cause of economic losses for the swine industry and ranks as a primary contributor to mortality in swine. Over the last 20 years, as swine production operations have continued to consolidate, respiratory disease in swine has become increasingly multifactorial in nature. The work presented in this dissertation centers around the development of vaccines utilizing the baculovirus expression vector system (BEVS) for two viral pathogens associated with the Porcine Respiratory Disease Complex (PRDC), a multifactorial respiratory disease syndrome affecting growing and finishing phase pigs. The work in this dissertation demonstrates that vaccines formulated with subunit hemagglutinin (HA) fused to an IgG Fc domain, VLP-displayed HA, or baculovirus-displayed HA may be viable alternatives to the traditional inactivated whole virus approaches currently utilized in the veterinary vaccine industry. In addition, this work uncovers a potential problem for the development of VLP-based porcine circovirus type 2 (PCV2) vaccines as an ORF2 protein from an emerging PCV2b strain was found to have dramatically reduced yields of VLP when expressed using the BEVS. Furthermore, the work in this dissertation demonstrates that a single amino acid mutation can be used to overcome this loss of VLP yield and that the mutated ORF2 protein provides protective efficacy against a PCV2b challenge in pigs.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/14390/
dc.identifier.articleid 5397
dc.identifier.contextkey 7907274
dc.identifier.doi https://doi.org/10.31274/etd-180810-3942
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/14390
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/28575
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/14390/Hernandez_iastate_0097E_14818.pdf|||Fri Jan 14 20:19:20 UTC 2022
dc.subject.disciplines Veterinary Medicine
dc.subject.disciplines Virology
dc.subject.keywords Veterinary Microbiology
dc.subject.keywords circovirus
dc.subject.keywords hemagglutinin
dc.subject.keywords influenza
dc.subject.keywords ORF2
dc.subject.keywords swine
dc.subject.keywords VLP
dc.title Successes and challenges in the developmemt of baculovirus-based vaccines for swine influenza and porcine circovirus type 2b
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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