Rapid Communication: Mapping of an X-Linked Porcine Microsatellite

Date
1996
Authors
Kapke, Paul
Wales, R.
Siggens, K.
Plastow, G.
Rothschild, Max
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Altmetrics
Authors
Research Projects
Organizational Units
Animal Science
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Abstract

Source and Description of Probe. Microsatellite locus S0511, originally identified from a porcine genomic library screened with d(CA)9, was used in these studies. Primers MS6F: 5¢AAAAACACGGAA GCAATAGAAATGTC-3¢ and MS5R: 5¢-GTCCATC CATGTTGCTCCAAATGG-3¢ were used in a PCR protocol to amplify sequence specific alleles. Primer MS6F was labeled using [g-32P]dATP (3,000 Ci/mmol, DuPont NEN) and polynucleotide kinase (New England Biolabs).

Description

This is an article from Journal of Animal Science 74 (1996): 1442, doi:/1996.7461442x. Posted with permission.

Keywords
Porcine, Dinucleotide Repeat, Microsatellite, PCR, X-linked
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