The maize brown midrib2 (bm2) gene encodes a methylenetetrahydrofolate reductase that contributes to lignin accumulation

dc.contributor.author Tang, Ho Man
dc.contributor.author Nettleton, Dan
dc.contributor.author Liu, Sanzhen
dc.contributor.author Hill-Skinner, Sarah
dc.contributor.author Wu, Wei
dc.contributor.author Reed, Danielle
dc.contributor.author Yeh, Cheng-Ting
dc.contributor.author Nettleton, Dan
dc.contributor.author Schnable, Patrick
dc.contributor.department Statistics
dc.contributor.department Agronomy
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Center for Plant Genomics
dc.date 2019-08-22T08:00:37.000
dc.date.accessioned 2020-07-02T06:57:03Z
dc.date.available 2020-07-02T06:57:03Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2014-02-01
dc.description.abstract <p>The midribs of maize <em>brown midrib</em> (<em>bm</em>) mutants exhibit a reddish‐brown color associated with reductions in lignin concentration and alterations in lignin composition. Here, we report the mapping, cloning, and functional and biochemical analyses of the <em>bm2</em> gene. The <em>bm2</em> gene was mapped to a small region of chromosome 1 that contains a putative methylenetetrahydrofolate reductase (MTHFR) gene, which is down‐regulated in <em>bm2</em> mutant plants. Analyses of multiple <em>Mu</em>‐induced <em>bm2‐Mu</em> mutant alleles confirmed that this constitutively expressed gene is <em>bm2</em>. Yeast complementation experiments and a previously published biochemical characterization show that the <em>bm2</em> gene encodes a functional MTHFR. Quantitative RT<em>‐</em>PCR analyses demonstrated that the <em>bm2</em> mutants accumulate substantially reduced levels of <em>bm2</em> transcript. Alteration of MTHFR function is expected to influence accumulation of the methyl donor <em>S‐</em>adenosyl‐l<em>‐</em>methionine (SAM). Because SAM is consumed by two methyltransferases in the lignin pathway (Ye <em>et al</em>., <a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12394#tpj12394-bib-0056" id="x-x-tpj12394-bib-0056R">1994</a>), the finding that <em>bm2</em> encodes a functional MTHFR is consistent with its lignin phenotype. Consistent with this functional assignment of <em>bm2</em>, the expression patterns of genes in a variety of SAM‐dependent or ‐related pathways, including lignin biosynthesis, are altered in the <em>bm2</em> mutant. Biochemical assays confirmed that <em>bm2</em> mutants accumulate reduced levels of lignin with altered composition compared to wild‐type. Hence, this study demonstrates a role for MTHFR in lignin biosynthesis.</p>
dc.description.comments <p>This is an article published as Tang, Ho Man, Sanzhen Liu, Sarah Hill‐Skinner, Wei Wu, Danielle Reed, Cheng‐Ting Yeh, Dan Nettleton, and Patrick S. Schnable. "The maize brown midrib2 (bm2) gene encodes a methylenetetrahydrofolate reductase that contributes to lignin accumulation." <em>The Plant Journal</em> 77, no. 3 (2014): 380-392. doi: <a href="https://doi.org/10.1111/tpj.12394">10.1111/tpj.12394</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/stat_las_pubs/189/
dc.identifier.articleid 1197
dc.identifier.contextkey 14824522
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath stat_las_pubs/189
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/90497
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/stat_las_pubs/189/2014_Nettleton_MaizeBrown.pdf|||Fri Jan 14 21:47:40 UTC 2022
dc.source.uri 10.1111/tpj.12394
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Computational Biology
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Zea mays
dc.subject.keywords brown midrib
dc.subject.keywords lignin
dc.subject.keywords methylenetetrahydrofolate
dc.subject.keywords S-adenosyl-L-methionine
dc.title The maize brown midrib2 (bm2) gene encodes a methylenetetrahydrofolate reductase that contributes to lignin accumulation
dc.type article
dc.type.genre article
dspace.entity.type Publication
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