Identification and characterization of a toxin-antitoxin system in the pVir plasmid of Campylobacter jejuni

dc.contributor.advisor Qijing Zhang
dc.contributor.author Patil, Rocky
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-08-11T15:25:46.000
dc.date.accessioned 2020-06-30T02:27:28Z
dc.date.available 2020-06-30T02:27:28Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.embargo 2013-06-05
dc.date.issued 2011-01-01
dc.description.abstract <p>Toxin-antitoxin systems are prevalent in different bacterial organisms and are encoded in the chromosomal or plasmid DNA. The primary function of a plasmid toxin-antitoxin module is to stabilize the plasmid by eliminating plasmid-free daughter cells through a post segregation killing mechanism. In Campylobacter jejuni, a pathogen that causes human gastroenteritis, multiple plasmids have been identified, but there have not been any reports on toxin-antitoxin systems. In this study, a toxin-antitoxin system is identified and characterized in the pVir plasmid of C. jejuni IA 3902. pVir encodes a type IV secretion system and is found to be involved in natural transformation and virulence in Campylobacter. Comparative genomic analysis of the pVir45 and pVir46 genes in C. jejuni indicates that they encode a putative toxin-antitoxin system that belongs to the RelE/StbE family. Cloning and expression of the pVir46 gene alone in Escherichia coli inhibited bacterial growth, but co-expression of pVir45-46 led to the restoration of growth, confirming the functions deduced from comparative genomic analysis. The plasmid stability assay in C. jejuni showed that the toxin-antitoxin system is necessary for maintaining the stability of pVir because deletion of the pVir46 gene resulted in loss of the plasmid during passage in conventional media. qRT-PCR data demonstrated that expression of the pVir45-46 genes varied with growth phase, as early logarithmic phase had the highest level of gene expression. Together these results establish that pVir45-46 encode a functional toxin-antitoxin system in C. jejuni, which is required for ensuring the stability of the pVir plasmid. The stable maintenance of pVir may be necessary for optimal virulence of C. jejuni IA 3902.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/10297/
dc.identifier.articleid 1212
dc.identifier.contextkey 2736309
dc.identifier.doi https://doi.org/10.31274/etd-180810-5677
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/10297
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24514
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/10297/Patil_iastate_0097M_12282.pdf|||Fri Jan 14 18:17:44 UTC 2022
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Plasmid Stability
dc.subject.keywords Toxin-Antitoxin System
dc.title Identification and characterization of a toxin-antitoxin system in the pVir plasmid of Campylobacter jejuni
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
thesis.degree.level thesis
thesis.degree.name Master of Science
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