Developmental proteomics in soybean embryos

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2002-01-01
Authors
Flack, Andrea
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In soybeans, incoming carbon is fixed into the three major storage products of protein, oil, and carbohydrates. However, little is known about control of the partitioning of that carbon. Interdisciplinary research will be conducted on various levels, including mRNA, proteins, and metabolites, to elucidate the control of carbon partitioning. The use of a parent and nearly isogenic lines of soybeans will offer insight into key points in the regulation of carbon partitioning. This study includes the use of proteomics in the parent line of Evans. Proteomics, was done using standard two-dimensional gel electrophoresis. Early stages of seed development were studied in which thirty-seven different protein expression patterns were generated from the 1551 total protein spots present. A total of 990 protein spots were excised from the gel for identification. There were 225 spots that were sampled multiple times. One hundred eight spots offered identical identifications. Of the remaining 117 spots, 56 had one or more poor putative identifications, 49 lacked any peptide mass fingerprint homology, and 12 had a combination of poor and non-existent identifications. The remaining 624 were given putative identifications. The reproducibility of protein spot presence in the two dimensional gel was tested. It was found that of the 691 total spots present in the 13 days after flowering (DAF) sample, 23.2% were present in two or less while 76.8% were present in three or more of the five total gels chosen the represent the time point. In 17 DAF, 712 total protein spots were detected with 9.5% present in two or less gels and 90.5% present in three or more of the five total gels chosen to represent the sample. In 21 DAY, 725 total protein spots were detected with 4% present in two or less gels and 96% in three or more of the five total gels chosen to represent the sample. For 24 DAF, a total of 780 protein spots were detected with 9.9% being present in two or less gels and 90.1 % in three or more of the five total gels chosen to represent the sample.

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Botany, Plant physiology
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