Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells

dc.contributor.author Saiyasombat, Rungrat
dc.contributor.author Carrillo-Tripp, Jimena
dc.contributor.author Miller, W. Allen
dc.contributor.author Bredenbeek, Peter
dc.contributor.author Blitvich, Bradley
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-02-17T04:45:29.000
dc.date.accessioned 2020-06-30T06:23:39Z
dc.date.available 2020-06-30T06:23:39Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-01-01
dc.description.abstract <p>Background: Most known flaviviruses, including West Nile virus (WNV), are maintained in natural transmission cycles between hematophagous arthropods and vertebrate hosts. Other flaviviruses such as Modoc virus (MODV) and Culex flavivirus (CxFV) have host ranges restricted to vertebrates and insects, respectively. The genetic elements that modulate the differential host ranges and transmission cycles of these viruses have not been identified. Methods: Fusion polymerase chain reaction (PCR) was used to replace the capsid (C), premembrane (prM) and envelope (E) genes and the prM-E genes of a full-length MODV infectious cDNA clone with the corresponding regions of WNV and CxFV. Fusion products were directly transfected into baby hamster kidney-derived cells that stably express T7 RNA polymerase. At 4 days post-transfection, aliquots of each supernatant were inoculated onto vertebrate (BHK-21 and Vero) and mosquito (C6/36) cells which were then assayed for evidence of viral infection by reverse transcription-PCR, Western blot and plaque assay. Results: Chimeric virus was recovered in cells transfected with the fusion product containing the prM-E genes of WNV. The virus could infect vertebrate but not mosquito cells. The in vitro replication kinetics and yields of the chimeric virus were similar to MODV but the chimeric virus produced larger plaques. Chimeric virus was not recovered in cells transfected with any of the other fusion products. Conclusions: Our data indicate that genetic elements outside of the prM-E gene region of MODV condition its vertebrate-specific phenotype.</p>
dc.description.comments <p>This article is from <em>Virology Journal</em> 11 (2014): 150, doi: <a href="http://dx.doi.org/10.1186/1743-422X-11-150" target="_blank">10.1186/1743-422X-11-150</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/43/
dc.identifier.articleid 1049
dc.identifier.contextkey 7791580
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/43
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57760
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/43/2014_MillerWA_SubstitutionPremembraneEnvelope.pdf|||Sat Jan 15 00:14:56 UTC 2022
dc.source.uri 10.1186/1743-422X-11-150
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Microbiology
dc.subject.disciplines Plant Pathology
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.keywords Modoc virus
dc.subject.keywords West Nile virus
dc.subject.keywords Culex flavivirus
dc.subject.keywords Chimeric flavivirus
dc.subject.keywords Fusion PCR
dc.title Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 4ad3ad12-430c-43b9-8f3c-3a920e00b28c
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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