T Lymphocyte Reactivity to Glutamic Acid-Alanine-Tyrosine In Vitro Does Not Reflect Antibody Response In Vivo

dc.contributor.author Steadham, Edward
dc.contributor.author Lamont, Susan
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2021-04-15T17:52:00.000
dc.date.accessioned 2021-04-29T23:53:29Z
dc.date.available 2021-04-29T23:53:29Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 1993
dc.date.issued 1993
dc.description.abstract <p><p id="x-x-spara10">Mechanisms responsible for the differences in humoral immune response to GAT (a random linear amino acid polymer) were investigated in a line of chickens consisting of four sublines homozygous for <em>Ea-B</em> (<em>B</em>1 or <em>B</em>19) and high or low antibody response to GAT (Ir-GATH or Ir-GATL). Previous research provided evidence of chromosomal recombination between the serologically determined regions of the MHC (encoded by <em>B</em>-<em>F</em> and <em>B</em>-<em>G</em> genes) and the gene or genes that control immune response to GAT, but immune response to GAT did not seem to be mediated through differences in <em>B-L</em> gene products. In the present study, proliferation of GAT-primed T lymphocytes indicated that reactivity <em>in vitro</em> was not associated with antibody levels produced in the animal. Cell surface markers were identified by flow cytometry. Lymphocytes from <em>Ea-B</em>19 chickens that were Ir-GATL had a higher percentage of suppressor T (CD8)-positive cells than did lymphocytes from Ir-GATH chickens. The <em>Ea-B</em>1 chickens that were Ir-GATL had a higher percentage of CD4-positive lymphocytes than did chickens that were Ir-GATH. This may indicate that low response to GAT in the <em>Ea-B</em>19 chickens, but not in <em>Ea-B</em>1 chickens, is mediated by CD8-positive cells. The ability of antigen-presenting cells (APC) to process and present GAT to antigen-primed T lymphocytes was tested <em>in vitro.</em> Measurements of lymphocyte proliferation indicated that, within the <em>Ea-B</em>1 blood type, APC from Ir-GATL chickens produced higher (<em>P</em> < .05) stimulation of both GAT low- and GAT high-responder lymphocytes. It is possible that, between the two <em>B</em> blood types, there are different mechanisms responsible for the differential response to GAT.</p>
dc.description.comments <p>This article is published as Steadham, E. M., and S. J. Lamont. "T lymphocyte reactivity to Glutamic Acid-Alanine-Tyrosine in vitro does not reflect antibody response in vivo." <em>Poultry Science</em> 72, no. 1 (1993): 67-75. DOI: <a href="https://doi.org/10.3382/ps.0720067" target="_blank">10.3382/ps.0720067</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/842/
dc.identifier.articleid 1840
dc.identifier.contextkey 22489049
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/842
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/104596
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/842/1993_LamontSusan_TLymphocyte.pdf|||Sat Jan 15 02:11:28 UTC 2022
dc.source.uri 10.3382/ps.0720067
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords immune response
dc.subject.keywords glutamic acid-alanine-tyrosine
dc.subject.keywords genetic differences
dc.subject.keywords chickens
dc.subject.keywords major histocompatibility complex
dc.title T Lymphocyte Reactivity to Glutamic Acid-Alanine-Tyrosine In Vitro Does Not Reflect Antibody Response In Vivo
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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