Development of fluorescence-based methods for non-invasive measurement of integrin microclustering
dc.contributor.advisor | Emily A. Smith | |
dc.contributor.author | Dibya, Deepak | |
dc.contributor.department | Chemistry | |
dc.date | 2018-08-11T09:15:32.000 | |
dc.date.accessioned | 2020-06-30T02:37:15Z | |
dc.date.available | 2020-06-30T02:37:15Z | |
dc.date.copyright | Fri Jan 01 00:00:00 UTC 2010 | |
dc.date.embargo | 2013-06-05 | |
dc.date.issued | 2010-01-01 | |
dc.description.abstract | <p>In order to better understand the cell signaling process, non-invasive methods to measure the integrin microclustering and examine the factors affecting the dynamics of these clusters are needed. This dissertation is focused on the development of non-invasive analytical tools to investigate the role of cytoplasmic proteins and cholesterol on the nanoscale dynamic localization of integrins using a technique called FRET, fluorescence resonance energy transfer. FRET is used to detect the proximity of molecules at nanometer distances (1-10 nm) and can be applied in diverse ways with various microscope configurations. FRET images obtained with such setups can be used to quantify the interactions between biomolecules within cells.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/etd/11659/ | |
dc.identifier.articleid | 2703 | |
dc.identifier.contextkey | 2807901 | |
dc.identifier.doi | https://doi.org/10.31274/etd-180810-2142 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | etd/11659 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/25865 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/etd/11659/Dibya_iastate_0097E_10999.pdf|||Fri Jan 14 18:55:11 UTC 2022 | |
dc.subject.disciplines | Chemistry | |
dc.subject.keywords | CHOLESTROL | |
dc.subject.keywords | FLUORESCENCE | |
dc.subject.keywords | FRET | |
dc.subject.keywords | INTEGRINS | |
dc.subject.keywords | MEMBRANE ORGANIZATION | |
dc.subject.keywords | MICROCLUSTERING | |
dc.title | Development of fluorescence-based methods for non-invasive measurement of integrin microclustering | |
dc.type | article | |
dc.type.genre | dissertation | |
dspace.entity.type | Publication | |
relation.isOrgUnitOfPublication | 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11 | |
thesis.degree.level | dissertation | |
thesis.degree.name | Doctor of Philosophy |
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