Analysis of B-G and Immune Response Genes in the Iowa State University S1 Chicken Line by Hybridization of Sperm Deoxyribonucleic Acid with a Major Histocompatibility Complex Class II Probe

Date
1989
Authors
Pitcovski, J.
Lamont, Susan
Nordskog, A. W.
Warner, C. M.
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Animal Science
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Abstract

Sperm DNA was isolated from chickens of the Iowa State University S1 line. Birds were from sublines selected for B-G antigen, humoral immune response to glutamic acid-alanine-tyrosine (IrGAT), and response to Rous sarcoma virus-induced (RSV) tumors. The DNA was digested with restriction enzymes and subjected to Southern blot analysis with a DNA probe specific for a class II gene of the chicken major histocompatibility complex (MHC).

Restriction fragment length polymorphisms (RFLP) associated with B-G antigen type were found after digestion of DNA with three enzymes, PvuII, BglII, or Sau3A, out of a total of 15 tested. No RFLP were shown to be associated with IrGAT or RSV type. This study shows that RFLP analysis of DNA may be a useful addition to or alternative to serological evaluation of MHC haplotype in the chicken.

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This article is published as Pitcovski, J., S. J. Lamont, A. W. Nordskog, and C. M. Warner. "Analysis of BG and immune response genes in the Iowa State University S1 chicken line by hybridization of sperm deoxyribonucleic acid with a major histocompatibility complex class II probe." Poultry Science 68, no. 1 (1989): 94-99. DOI: 10.3382/ps.0680094. Posted with permission.

Keywords
major histocompatibility complex, class II DNA probe, restriction fragment, length polymorphism
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