Monarch larvae sensitivity to Bacillus thuringiensispurified proteins and pollen

dc.contributor.author Hellmich, Richard
dc.contributor.author Hellmich, Richard
dc.contributor.author Siegfried, Blair
dc.contributor.author Sears, Mark
dc.contributor.author Stanley-Horn, Diane
dc.contributor.author Daniels, Michael
dc.contributor.author Mattila, Heather
dc.contributor.author Spencer, Terrence
dc.contributor.author Bidne, Keith
dc.contributor.author Lewis, Leslie
dc.contributor.department Entomology
dc.date 2018-02-14T10:19:18.000
dc.date.accessioned 2020-06-30T02:25:25Z
dc.date.available 2020-06-30T02:25:25Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2001
dc.date.embargo 2014-08-20
dc.date.issued 2001-10-01
dc.description.abstract <p>Laboratory tests were conducted to establish the relative toxicity of Bacillus thuringiensis (Bt) toxins and pollen from Bt corn to monarch larvae. Toxins tested included Cry1Ab, Cry1Ac, Cry9C, and Cry1F. Three methods were used: (i) purified toxins incorporated into artificial diet, (ii) pollen collected from Bt corn hybrids applied directly to milkweed leaf discs, and (iii) Bt pollen contaminated with corn tassel material applied directly to milkweed leaf discs. Bioassays of purified Bt toxins indicate that Cry9C and Cry1F proteins are relatively nontoxic to monarch first instars, whereas first instars are sensitive to Cry1Ab and Cry1Ac proteins. Older instars were 12 to 23 times less susceptible to Cry1Ab toxin compared with first instars. Pollen bioassays suggest that pollen contaminants, an artifact of pollen processing, can dramatically influence larval survival and weight gains and produce spurious results. The only transgenic corn pollen that consistently affected monarch larvae was from Cry1Ab event 176 hybrids, currently<2%corn planted and for which re-registration has not been applied. Results from the other types of Bt corn suggest that pollen from the Cry1Ab (events Bt11 and Mon810) and Cry1F, and experimental Cry9C hybrids, will have no acute effects on monarch butterfly larvae in field settings.</p>
dc.description.comments <p>This article is from <em>Proceedings of the National Academy of Sciences USA</em>; 98 (2001); 11925-11930; doi: <a href="http://dx.doi.org/10.1073ypnas.211297698" target="_blank">10.1073ypnas.211297698</a></p>
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dc.identifier archive/lib.dr.iastate.edu/ent_pubs/69/
dc.identifier.articleid 1078
dc.identifier.contextkey 6010800
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/69
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24227
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/69/2001_HellmichRL_MonarchLarvaeSensitivity.pdf|||Sat Jan 15 01:30:10 UTC 2022
dc.source.uri 10.1073ypnas.211297698
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Entomology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Department of Environmental Biology
dc.subject.keywords Corn Insects and Plant Genetics Research Unit
dc.subject.keywords Department of Environmental Biology
dc.title Monarch larvae sensitivity to Bacillus thuringiensispurified proteins and pollen
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3cca44d8-e1df-437d-a95a-d8e38963d2c1
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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