Telomere G-strand structure and function analyzed by chemical protection, base analog substitution, and utilization by telomerase in vitro

dc.contributor.author Henderson, Eric
dc.contributor.author Moore, Michael
dc.contributor.author Henderson, Eric
dc.contributor.author Malcolm, Bruce
dc.contributor.department Zoology and Genetics
dc.date 2018-02-18T09:56:02.000
dc.date.accessioned 2020-07-07T05:16:56Z
dc.date.available 2020-07-07T05:16:56Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 1990
dc.date.issued 1990
dc.description.abstract <p>Eukaryotic telomeres have a 12-1 6 nucleotide long deoxyguanosine (dG) rich single-stranded overhang at their molecular termini. Some of the unique features of telomeres are probably attributable to a specialized structure formed by this overhang. In the ciliated protozoan Tetrahymena thermophila, the dG-rich overhang is comprised of approximately two repeats of the sequence d(TTGGGG). Previous work has shown that the synthetic oligonucleotide d(TTGGGG),, can form an unusual non-Watson-Crick base-paired structure (the "G-strand structure") containing G-G base pairs and syn-guanines. We have tested the susceptibility of various dGs in this structure to methylation by DMS. At 0-10 "C one dG residue is hypersensitive to methylation while others are particularly resistant. By systematically substituting deoxyinosine (dI) for dG in d(TTGGGG)4 we identify N2 groups of guanine essential for formation of the G-strand structure. We show that dI-substituted molecules that cannot form the G-strand structure nonetheless function as substrates for telomere repeat addition in vitro by the telomere lengthening enzyme, telomerase. The implications of these data are discussed.</p>
dc.description.comments <p>Reprinted (adapted) with permission from Telomere G-strand structure and function analyzed by chemical protection, base analog substitution, and utilization by telomerase in vitro. Eric R. Henderson, Michael Moore, and Bruce A. Malcolm. Biochemistry <strong>1990</strong> <em>29</em> (3), 732-737. DOI: 10.1021/bi00455a020. Copyright 1990 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/38/
dc.identifier.articleid 1032
dc.identifier.contextkey 10135389
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/38
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92648
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/38/0-Permission_for_ACS_1990_Telomere.pdf|||Fri Jan 14 23:52:24 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/38/1990_Henderson_TelomereG.pdf|||Fri Jan 14 23:52:26 UTC 2022
dc.source.uri 10.1021/bi00455a020
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.title Telomere G-strand structure and function analyzed by chemical protection, base analog substitution, and utilization by telomerase in vitro
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 68bc511a-afd6-4353-ae20-066de33682b9
relation.isOrgUnitOfPublication 4a2929da-5374-4338-b62f-f5fd9e156ef9
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