Involvement of protein degradation, calpain autolysis and protein nitrosylation in fresh meat quality during early postmortem refrigerated storage

dc.contributor.advisor Elisabeth Huff-lonergan
dc.contributor.author Zhang, Wangang
dc.contributor.department Animal Science
dc.date 2018-08-11T13:24:08.000
dc.date.accessioned 2020-06-30T02:29:47Z
dc.date.available 2020-06-30T02:29:47Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2009
dc.date.embargo 2013-06-05
dc.date.issued 2009-01-01
dc.description.abstract <p>Fresh meat quality including meat tenderness and water holding capacity are important for consumer acceptance and industry profitability in the US. Exploring the basic mechanism that regulates the development of meat quality attributes is critical for delivering consistently high quality fresh meat to consumers. The overall hypothesis of this study was that biochemical factors that influence proteolysis during the antemortem or the early postmortem period can contribute to variations in fresh meat quality. The objective was to determine the extent to which protein modifications including protein degradation, protein nitrosylation and calpain autolysis affect the development of fresh meat quality.</p> <p>The first experiment was designed to test the hypothesis that protein degradation and calpain activation could influence water holding capacity of fresh pork. Intensity of intact desmin was positively correlated with moisture loss, while intensity of intact integrin was negatively correlated with moisture loss during postmortem storage. Significant correlations were found between different calpain autolysis products and intensity of intact desmin and moisture loss. These results support the hypothesis that proteolysis of desmin contributes to greater water holding capacity. The second experiment hypothesized that the properties of calpains could be regulated by a nitric oxide donor S-nitrosoglutathione. S-Nitrosoglutathione could further nitrosylate y-calpain in both the absence and the presence of calcium especially at pH 6.5. The combination of S-nitrosoglutathione and calcium affected the activity of m- and y-calpain and regulated the rate of y-calpain autolysis. In the third experiment, the hypothesis that the muscle fiber type could be related to the levels of protein nitrosylation in postmortem beef was tested. Beef longissimus dorsi muscle showed higher percentage of type IIA/X and lower percentage of type I myosin heavy chain isoforms than psoas major muscle. The intensity of protein nitrosylation in longissimus dorsi muscle was greater compared to psoas major muscle in samples prepared 1 d postmortem. In conclusion, biochemical and biophysical reactions during preslaughter and postmortem storage can mediate the development of fresh meat quality during postmortem aging.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/10623/
dc.identifier.articleid 1612
dc.identifier.contextkey 2806779
dc.identifier.doi https://doi.org/10.31274/etd-180810-2884
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/10623
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24829
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/10623/Zhang_iastate_0097E_10461.pdf|||Fri Jan 14 18:25:00 UTC 2022
dc.subject.disciplines Animal Sciences
dc.subject.keywords autolysis
dc.subject.keywords calpain
dc.subject.keywords drip loss
dc.subject.keywords pork
dc.subject.keywords protein degradation
dc.subject.keywords protein nitrosylation
dc.title Involvement of protein degradation, calpain autolysis and protein nitrosylation in fresh meat quality during early postmortem refrigerated storage
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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