Interdependent evolution of biosynthetic gene clusters for momilactone production in rice

dc.contributor.author Kitaoka, Naoki
dc.contributor.author Zhang, Juan
dc.contributor.author Peters, Reuben
dc.contributor.author Oyagbenro, Richard
dc.contributor.author Brown, Benjamin
dc.contributor.author Wu, Yisheng
dc.contributor.author Yang, Bing
dc.contributor.author Li, Zhaohu
dc.contributor.author Peters, Reuben
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2021-04-29T13:00:31.000
dc.date.accessioned 2021-04-29T23:57:50Z
dc.date.available 2021-04-29T23:57:50Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.embargo 2022-02-01
dc.date.issued 2021-02-01
dc.description.abstract <p>Plants can contain biosynthetic gene clusters (BGCs) that nominally resemble those found in microbes. However, while horizontal gene transmission is often observed in microbes, plants are limited to vertical gene transmission, implying that their BGCs may exhibit distinct inheritance patterns. Rice (Oryza sativa) contains two unlinked BGCs involved in diterpenoid phytoalexin metabolism, with one clearly required for momilactone biosynthesis, while the other is associated with production of phytocassanes. Here, in the process of elucidating momilactone biosynthesis, genetic evidence was found demonstrating a role for a cytochrome P450 (CYP) from the other ‘phytocassane’ BGC. This CYP76M8 acts after the CYP99A2/3 from the ‘momilactone’ BGC, producing a hemiacetal intermediate that is oxidized to the eponymous lactone by a short-chain alcohol dehydrogenase also from this BGC. Thus, the ‘momilactone’ BGC is not only incomplete, but also fractured by the need for CYP76M8 to act in between steps catalyzed by enzymes from this BGC. Moreover, as supported by similar activity observed with orthologs from the momilactone-producing wild-rice species Oryza punctata, the presence of CYP76M8 in the other ‘phytocassane’ BGC indicates interdependent evolution of these two BGCs, highlighting the distinct nature of BGC assembly in plants.</p>
dc.description.comments <p>This is a manuscript of an article published as Kitaoka, Naoki, Juan Zhang, Richard K. Oyagbenro, Benjamin Brown, Yisheng Wu, Bing Yang, Zhaohu Li, and Reuben J. Peters. "Interdependent evolution of biosynthetic gene clusters for momilactone production in rice." <em>The Plant Cell</em> 33 (2020): 290-305. doi:<a href="https://doi.org/10.1093/plcell/koaa023">10.1093/plcell/koaa023</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/308/
dc.identifier.articleid 1316
dc.identifier.contextkey 22709418
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/308
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/104638
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/308/2020_Peters_InterdependentEvolutionManuscript.pdf|||Fri Jan 14 23:29:34 UTC 2022
dc.source.uri 10.1093/plcell/koaa023
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Evolution
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Sciences
dc.title Interdependent evolution of biosynthetic gene clusters for momilactone production in rice
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 498a24ec-81d7-4bee-b145-323d38e7a392
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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