Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7

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2011-11-04
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Wu, Yinsheng
Hillwig, Matthew
Wang, Qiang
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Peters, Reuben
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Abstract

Rice (Oryza sativa) contains a biosynthetic gene cluster associated with production of at least two groups of diterpenoid phytoalexins, the antifungal phytocassanes and antibacterial oryzalides. While cytochromes P450 (CYP) from this cluster are known to be involved in phytocassane production, such mono-oxygenase activity relevant to oryzalide biosynthesis was unknown. Here we report biochemical characterization demonstrating that CYP71Z6 from this cluster acts as an ent-isokaurene C2-hydroxylase that is presumably involved in the biosynthesis of oryzalides. Our results further suggest that the closely related and co-clustered CYP71Z7 likely acts as a C2- hydroxylase involved in a latter step of phytocassane biosynthesis. Thus, CYP71Z6 & 7 appear to have evolved distinct roles in rice diterpenoid metabolism, offering insight into plant biosynthetic gene cluster evolution.

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This is the peer reviewed version of the following article: Wu Yisheng,Hillwig Matthew L.,Wang Qiang and Peters Reuben J.(2011), Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7, FEBS Letters, 585, which has been published in final form at doi: 10.1016/j.febslet.2011.09.038. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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Sat Jan 01 00:00:00 UTC 2011
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