Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative

Date
2017-03-01
Authors
Colonna, William
Marti, Mustafa
Nyman, John
Green, Chris
Glatz, Charles
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Center for Crops Utilization ResearchChemical and Biological Engineering
Abstract

If biosurfactants are to achieve their promise in environmental oil-spill remediation, their toxicity to marine life must be assessed. A killifish larvae assay is commonly used as a measure of toxicity but is difficult and nonlinear in response. Red blood cell (RBC) hemolysis has also been the basis for assays of some surfactant levels. Here we present a modified sheep RBC suspension assay and compare its response to that of the fish assay for surfactin and its genetically modified variant fatty-acyl-glutamate (FA-Glu). This is the first report of hemolytic activity as a property of FA-Glu. The method's potential for screening for toxicity against marine organisms is demonstrated.

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This is the peer reviewed version of the following article: Colonna, William J., Mustafa E. Martı, John A. Nyman, Chris Green, and Charles E. Glatz. "Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative." Environmental Progress & Sustainable Energy 36, no. 2 (2017): 505-510, which has been published in final form at 10.1002/ep.12444. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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