Mitigating Scoring Errors in Microsatellite Data from Wild Populations
Mitigating Scoring Errors in Microsatellite Data from Wild Populations
Date
2006-01-01
Authors
DeWoody, Jennifer
Nason, John
Nason, John
Hipkins, Valerie
Nason, John
Nason, John
Hipkins, Valerie
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Nason, John
Person
Research Projects
Organizational Units
Ecology, Evolution and Organismal Biology
Organizational Unit
Journal Issue
Series
Department
Ecology, Evolution and Organismal Biology
Abstract
Microsatellite data are widely used to test ecological and evolutionary hypotheses in wild populations. In this paper, we consider three typical sources of scoring errors capable of biasing biological conclusions: stuttering, large-allele dropout and null alleles. We describe methods to detect errors and propose conventions to mitigate scoring errors and report error rates in studies of wild populations. Finally, we discuss potential bias in ecological or evolutionary conclusions based on data sets containing these scoring errors.
Comments
This article is from Molecular Ecology Notes 6 (2006): 951, doi:10.1111/j.1471-8286.2006.01449.x.