Fatty acid elongase (FAE) systems: An investigation of genetic redundancy

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Campbell, Alexis
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Basil J. Nikolau
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Biochemistry, Biophysics and Molecular Biology

The Department of Biochemistry, Biophysics, and Molecular Biology was founded to give students an understanding of life principles through the understanding of chemical and physical principles. Among these principles are frontiers of biotechnology such as metabolic networking, the structure of hormones and proteins, genomics, and the like.

The Department of Biochemistry and Biophysics was founded in 1959, and was administered by the College of Sciences and Humanities (later, College of Liberal Arts & Sciences). In 1979 it became co-administered by the Department of Agriculture (later, College of Agriculture and Life Sciences). In 1998 its name changed to the Department of Biochemistry, Biophysics, and Molecular Biology.

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  • Department of Biochemistry and Biophysics (1959–1998)

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Biochemistry, Biophysics and Molecular Biology

Biosynthesis of very long chain fatty acids (VLCFAs) is an integral process for plants, animals & yeast. VLCFAs serve in a variety of biological processes (e.g. protein trafficking, membrane stabilization, precursors for lipid second messengers) and in plants, are components of cuticular waxes, suberin, sphingolipids, phospholipids, seed oils, and GPI anchors. VLCFAs are synthesized by the fatty acid elongase system (FAE), which is composed of four integral membrane proteins: 3-ketoacyl-CoA synthase (KCS), 3-ketoacyl-CoA reductase (KCR), 3-hydroxyacyl-CoA dehydratase (HCD), & an enoyl-CoA reductase using fatty acid primers provided by de novo fatty acid synthesis.

The study of FAE, particularly the role of paralogous genes encoding each of its catalytic components, can be difficult due to its integral membrane nature. We have therefore built a platform to study the FAE system in yeast: a more tractable system for functionally evaluating the enzymological role of each paralog. The paralogs include previously characterized maize KCRs (glossy8a and glossy8b) and in silico-identified maize genes encoding the remaining components of the system. We have shown each component gene can individually complement yeast lacking a functional copy of the ortholog, and are working towards the complete reconstitution of this FAE within a single yeast strain that lacks endogenous elongase. This strain series has been used to further our understanding of why maize retains two ZmKCR paralogs and understand their roles within the FAE multienzyme system. Additionally, these strains can now be used to complete the reconstitution of maize FAE in a system free of the endogenous yeast FAE. Bioengineering this system will not only be a powerful platform to characterize the interactions and functional significance of the FAE components, but also provides the biosynthetic machinery to produce long chain substrate for the functional characterization of the cuticular wax biosynthetic genes. Understanding this pathway is a useful tool for the production of potential candidates for next generation fuels.

Sat Jan 01 00:00:00 UTC 2011