Transposon Ac/Ds -induced chromosomal rearrangements at the rice OsRLG5 locus

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2011-09-28
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Xuan, Yuan Hu
Piao, Hai Long
Je, Byoung Il
Peterson, Thomas
Park, Soon Ju
Huang, Jin
Zhang, Jian Bo
Peterson, Thomas
Han, Chang-deok
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Abstract

Previous studies have shown that pairs of closely-linked Ac/Ds transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line ( OsRLG5-161) that contains two inversely-oriented Ds insertions in OsRLG5 ( Oryza sativa Receptor like kinase Gene 5). Among approximately 300 plants regenerated from OsRLG5-161 heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented Ds elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted Ds elements in OsRLG5-161 . The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual Ds termini. Together, these results demonstrate a greatly enlarged potential of the Ac/Ds system for plant chromosome engineering.

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<p>This article is from <em>Nucleic Acids Research </em>39 (2011): e149, doi: <a href="https://doi.org/10.1093/nar/gkr718">10.1093/nar/gkr718</a>. Posted with permission.</p>
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