Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

Clark, Natalie; Shen, Zhouxin; Briggs, Steven; Kelley, Dior; Walley, Justin; Kelley, Dior

Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

File

2019_Kelley_AuxinInducesManuscript.pdf (1.11 MB)

Date

2019-08-05

Authors

Clark, Natalie

Shen, Zhouxin

Briggs, Steven

Kelley, Dior

Walley, Justin

Kelley, Dior

Authors

Person

Kelley, Dior

Assistant Professor

Organizational Units

Organizational Unit

Plant Pathology and Microbiology

The Department of Plant Pathology and Microbiology and the Department of Entomology officially merged as of September 1, 2022. The new department is known as the Department of Plant Pathology, Entomology, and Microbiology (PPEM). The overall mission of the Department is to benefit society through research, teaching, and extension activities that improve pest management and prevent disease. Collectively, the Department consists of about 100 faculty, staff, and students who are engaged in research, teaching, and extension activities that are central to the mission of the College of Agriculture and Life Sciences. The Department possesses state-of-the-art research and teaching facilities in the Advanced Research and Teaching Building and in Science II. In addition, research and extension activities are performed off-campus at the Field Extension Education Laboratory, the Horticulture Station, the Agriculture Engineering/Agronomy Farm, and several Research and Demonstration Farms located around the state. Furthermore, the Department houses the Plant and Insect Diagnostic Clinic, the Iowa Soybean Research Center, the Insect Zoo, and BugGuide. Several USDA-ARS scientists are also affiliated with the Department.

Organizational Unit

Genetics, Development and Cell Biology

Department

Plant Pathology and MicrobiologyGenetics, Development and Cell Biology

Abstract

It is known that auxin induces rapid gene expression changes throughout plant development, but how these transcriptional responses relate to changes in protein abundance is not well characterized. This report identifies early auxin responsive proteins in whole Arabidopsis seedlings using an iTRAQ (isobaric Tags for Relative and Absolute Quantification)-based quantitative proteomics approach. Approximately 25% of the detected proteins (1045 out of 4257 proteins) were auxin responsive, which is in line with the central role of auxin in the regulation of plant growth and development. Several well-known auxin pathway proteins were identified as differentially expressed, validating this quantitative proteomics approach. Additionally, functional categorization of these auxin responsive proteins indicates that rapid and complex metabolic changes occur in seedlings in response to auxin, including lipid metabolism. Altogether, these data describe novel auxin-regulated proteins and are an excellent resource for identifying new downstream signaling components related to auxin-mediated plant growth and development.

Comments

This is the peer reviewed version of the following article: Clark, Natalie M., Zhouxin Shen, Steven P. Briggs, Justin W. Walley, and Dior R. Kelley. "Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings." Proteomics (2019), which has been published in final form at doi: 10.1002/pmic.201900199. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Copyright

Tue Jan 01 00:00:00 UTC 2019

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