A cyst nematode effector binds to diverse plant proteins, increases nematode susceptibility and affects root morphology

Pogorelko, Gennady
Baum, Thomas
Juvale, Parijat
Rutter, William
Hewezi, Tarek
Hussey, Richard
Davis, Eric
Mitchum, Melissa
Baum, Thomas
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Plant Pathology and MicrobiologyPlant Biology

Cyst nematodes are plant-parasitic roundworms that are of significance in many cropping systems around the world. Cyst nematode infection is facilitated by effector proteins secreted from the nematode into the plant host. The cDNAs of the 25A01-like effector family are novel sequences that were isolated from the esophageal gland cells of the soybean cyst nematode (Heterodera glycines). To aid functional characterization we identified an orthologous member of this protein family (Hs25A01) from the closely related sugar beet cyst nematode H. schachtii, which infects Arabidopsis. Constitutive expression of the Hs25A01 CDS in Arabidopsis plants caused a small increase in root length accompanied by up to 22% increase in susceptibility to H. schachtii. A plant-expressed RNAi construct targeting Hs25A01 transcripts in invading nematodes significantly reduced host susceptibility to H. schachtii. These data document that Hs25A01 has physiological functions in planta and a role in cyst nematode parasitism. In vivo as well as in vitro binding assays confirmed specific interactions of Hs25A01 with an Arabidopsis F-box containing protein, a chalcone synthase, and the translation initiation factor eIF- 2 beta subunit (eIF-2bs), making these proteins likely candidates for being involved in the observed changes in plant growth and parasitism. A role of eIF- 2bs in mediating the Hs25A01 virulence function is further supported by the observation that two independent eIF-2bs Arabidopsis knock-out lines were significantly more susceptible to H. schachtii.


This is the peer reviewed version of the following article: Pogorelko, Gennady, Parijat S. Juvale, William B. Rutter, Tarek Hewezi, Richard Hussey, Eric L. Davis, Melissa G. Mitchum, and Thomas J. Baum. "A cyst nematode effector binds to diverse plant proteins, increases nematode susceptibility and affects root morphology." Molecular plant pathology 17, no. 6 (2016): 832-844, which has been published in final form at DOI: 10.1111/mpp.12330. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.