Effect of dietary ethanol on atherosclerosis in rabbits

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Wilson, Edward
Major Professor
Richard L. Engen
Committee Member
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Veterinary Physiology and Pharmacology

The effect of dietary ethanol on atherosclerotic regression and vasoconstriction in atherosclerotic rabbits was addressed by feeding male New Zealand white rabbits (n = 19) a diet enriched with 0.8% cholesterol for 45 days, followed by randomization to one of three diets for the next 45 days: (1) Continued consumption of a cholesterol-rich diet (CC; n = 6), (2) Consumption of a cholesterol-free diet (CF; n = 6), and (3) Consumption of a cholesterol-free diet supplemented with ethanol in the drinking water (10% v/v; ECF; n = 7). Atherosclerosis and vasoconstrictive properties of the aortas were evaluated on day 90. Data are expressed as mean ± SEM, means with the same letter are not significantly (P < 0.05) different. Total plasma cholesterol of the CC, CF and ECF groups were 1532 ± 107.01[superscript] A, 440.50 ± 44.08[superscript] B and 957.79 ± 157.21 mg/dl[superscript] C, respectively. Plasma triglyceride concentrations of the CC, CF and ECF groups were 238.97 ± 64.28[superscript] A, 79.97 ± 11.77[superscript] A and 433.32 ± 122.07[superscript] B mg/dl, respectively. No significant differences were noted in regards to the percent of aortic surface covered by Sudan-IV staining lesions, fatty streak areas in aortic cross-sections, smooth muscle area or aortic cholesterol content. Neither the CF nor the ECF diets promoted atherosclerotic regression. The total liver cholesterol content of the CC, CF and ECF groups were 3.98 ± 0.33[superscript] A, 2.36 ± 0.30[superscript] B and 1.97 ± 0.31[superscript] B grams cholesterol/liver, respectively. Norepinephrine (10[superscript]-7M) promoted maximal contractile forces in the CC, CF and ECF groups of 3.69 ± 0.32[superscript] A, 5.09 ± 0.39[superscript] B and 5.31 ± 0.30[superscript] B grams, respectively. Potassium chloride (80 mM) promoted maximal contractile forces in the CC, CF and ECF groups of 4.01 ± 0.35[superscript] A, 5.04 ± 0.38[superscript] B and 5.34 ± 0.47 grams[superscript] B respectively. The contractile forces developed in response to 200 mg/dl ethanol by the CC, CF and ECF groups were 51.4 ± 7.5[superscript] A, 71.2 ± 12.6[superscript] A and 104.9 ± 31.5[superscript] A, respectively;Serotonin (10[superscript]-7M) developed contractile forces in the CC, CF and ECF groups of 2.35 ± 0.24[superscript] A, 2.13 ± 0.21[superscript] A and 2.89 ± 0.59[superscript] A grams, respectively. This investigation demonstrated that the inclusion of dietary ethanol as part of a lipid lowering diet in atherosclerotic rabbits is associated with the maintenance of risk factors promoting with vasospasm and continued atherogenesis.

Sat Jan 01 00:00:00 UTC 1994