The objective of this cross-sectional study was to analyze data available from multiple studies conducted by our research team estimating the prevalence of S. enterica, and the serotype d1stribution in swine at slaughter, based on different sample types A total of 1,110 pigs from three large capacity abattoirs located in the Midwestern U S. were individually sampled at slaughter. Individually paired samples collected mcluded. cecal contents and ileocecal lymph nodes. Samples were collected on multiple occasions in all three abattoirs, transported to the laboratory, and processed for the isolation and identification of S. enterica. The overall prevalence of S. enterica, based on cecal contents, mesentenc lymph nodes, and any of the samples (i.e., cecal contents and/or mesenteric lymph nodes) was 54.7%, 27.9%, and 62.6%, respectively. There was a Significant difference (P<0.05) between prevalence estimates based on cecal contents and mesenteric lymph node samples in all three abattoirs, and overall. A variety of S. enterica serotypes was isolated m all abattoirs The average number of serotypes isolated per group was 3.48. This study confirms that the S. entenca prevalence at slaughter in swine is high, requiring attention due to the associated risk of contamination of the abattoir environment. Moreover, our results demonstrate the common occurrence of a high diversity of serotypes in swine at slaughter. This study also shows that both cecal contents and mesenteric lymph nodes should be considered for a better estimate of S. enterica prevalence at slaughter.