Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling

Supplemental Files
Date
2018-12-05
Authors
Bao, Yan
Bassham, Diane
Bassham, Diane
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Journal Issue
Series
Department
Genetics, Development and Cell Biology
Abstract

Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol (DTT)-mediated root growth inhibition, analysis of splicing of the UPR-responsive transcription factor bZIP60 (basic Leucine Zipper Domain 60), and upregulation of relevant UPR genes. We provide here a quick and robust method to detect UPR signaling in Arabidopsis thaliana protoplasts. This assay can also be applied to other plant species for which protoplasts can be isolated.

Comments

This article is published Bao, Yan, and Diane C. Bassham. "Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling." Bio-protocol 8, no. 23 (2018) e3101. doi: 10.21769/BioProtoc.3101. Posted with permission.

Description
Keywords
Citation
DOI
Collections