The purpose of this study was to evaluate the teat end health and skin conditioning performance of an experimental “no mix” chlorine dioxide teat dip vs. the commercial dips and best management practices used at the ISU Dairy using a split udder design. The experimental chlorine dioxide dip showed similar, excellent teat skin health and conditioning compared to the herd’s dips and best management practices. However, average teat end scores and % rough teats were significantly higher in chlorine dioxide dipped teats, with this reaction occurring more on teats that had higher hyperkeratosis at the start. This localized, prolonged reaction on that subset of animals and teats should be evaluated and further rectified to assure similar excellent teat end health compared to industry standards and best management practices. There were also marked changes in scores and similar trends across groups and products signifying that other factors besides teat dips influence teat health.