Replication protein A subunit 3 and the iron efficiency response in soybean Atwood, Sarah O'Rourke, Jamie Yin, Tengfei Majumder, Mahbubul Whitham, Steven Zhang, Chunquan Cianzio, Sylvia Hill, John Cook, Dianne Whitham, Steven Shoemaker, Randy Graham, Michelle
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Statistics
dc.contributor.department Agronomy
dc.contributor.department Agronomy 2018-02-17T13:12:30.000 2020-06-30T06:23:53Z 2020-06-30T06:23:53Z 2014-01-01
dc.description.abstract <p>In soybean [Glycine max (L.) Merr.], iron deficiency results in interveinal chlorosis and decreased photosynthetic capacity, leading to stunting and yield loss. In this study,gene expression analyses investigated the role of soybean replication proteinA (RPA) subunits during iron stress. Nine RPA homologs were significantly differentially expressed in response to iron stress in the near isogenic lines (NILs) Clark (iron efficient) and Isoclark (iron inefficient).RPAhomologs exhibited opposing expression patterns in the two NILs, with RPA expression significantly repressed during iron deficiency in Clark but induced in Isoclark. We used virus induced gene silencing (VIGS) to repress GmRPA3 expression in the iron inefficient line Isoclark and mirror expression in Clark. GmRPA3- silenced plants had improved IDC symptoms and chlorophyll content under iron deficient conditions and also displayed stunted growth regardless of iron availability.RNA-Seq comparing gene expression between GmRPA3-silenced and empty vector plants revealed massive transcriptional reprogramming with differential expression of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis and iron uptake and transport genes. Our findings suggest the iron efficient genotype Clark is able to induce energy controlling pathways, possibly regulated by SnRK1/TOR, to promote nutrient recycling and stress responses in iron deficient conditions.</p>
dc.description.comments <p>This article is from <em>Plant, Cell and Environment</em> 37 (2014): 213, doi:<a href="" target="_blank">10.1111/pce.12147</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1074
dc.identifier.contextkey 8149947
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/78
dc.language.iso en
dc.source.bitstream archive/|||Sat Jan 15 01:54:07 UTC 2022
dc.source.uri 10.1111/pce.12147
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Pathology
dc.subject.keywords iron
dc.subject.keywords virus induced gene silencing
dc.title Replication protein A subunit 3 and the iron efficiency response in soybean
dc.type article
dc.type.genre article
dspace.entity.type Publication
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