Tryptophan and ATTO 590: Mutual Fluorescence Quenching and Exciplex Formation

dc.contributor.author Winter, Arthur
dc.contributor.author Bhattacharjee, Ujjal
dc.contributor.author Beck, Christie
dc.contributor.author Winter, Arthur
dc.contributor.author Petrich, Jacob
dc.contributor.author Wells, Carson
dc.contributor.author Petrich, Jacob
dc.contributor.department Ames Laboratory
dc.contributor.department Chemistry
dc.date 2018-02-17T06:44:36.000
dc.date.accessioned 2020-06-30T01:17:56Z
dc.date.available 2020-06-30T01:17:56Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-01-01
dc.description.abstract <p>Investigation of fluorescence quenching of probes, such as ATTO dyes, is becoming an increasingly important topic owing to the use of these dyes in super-resolution microscopies and in single-molecule studies. Photoinduced electron transfer is their most important nonradiative pathway. Because of the increasing frequency of the use of ATTO and related dyes to investigate biological systems, studies are presented for inter- and intramolecular quenching of ATTO 590 with tryptophan. In order to examine intramolecular quenching, an ATTO 590–tryptophan conjugate was synthesized. It was determined that tryptophan is efficiently quenching ATTO 590 fluorescence by excited-state charge transfer and two charge transfer complexes are forming. In addition, it was discovered that an exciplex (whose lifetime is 5.6 ns) can be formed between tryptophan and ATTO 590, and it is suggested that the possibility of such exciplex formation should be taken into account when protein fluorescence is monitored in a system tagged with ATTO dyes.</p>
dc.description.comments <p>Reprinted (adapted) with permission from J. Phys. Chem. B, 2014, 118 (29), pp 8471–8477. Copyright 2014 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/chem_pubs/147/
dc.identifier.articleid 1141
dc.identifier.contextkey 7886374
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/147
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/14581
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/147/0-2014_Winters_TryptophanATTO_License.pdf|||Fri Jan 14 20:24:54 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/147/2014_Winter_TryptophanATTO.pdf|||Fri Jan 14 20:24:56 UTC 2022
dc.source.uri 10.1021/jp412045m
dc.subject.disciplines Materials Chemistry
dc.subject.disciplines Other Chemistry
dc.subject.disciplines Physical Chemistry
dc.title Tryptophan and ATTO 590: Mutual Fluorescence Quenching and Exciplex Formation
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a2121da6-c591-494f-b9a9-595265e46ff9
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relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
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