Nematode CLE signaling in Arabidopsis requires CLAVATA2 and CORYNE

Date
2011-02-01
Authors
Replogle, Amy
Wang, Jianying
Bleckmann, Andrea
Hussey, Richard
Baum, Thomas
Sawa, Shinichiro
Davis, Eric
Wang, Xianhong
Simon, Rudiger
Mitchum, Melissa
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Abstract

Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR (CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors for nematode CLE-like peptides have not been identified. Here we demonstrate that CLV2 and CORYNE (CRN), members of the receptor kinase family, are required for nematode CLE signaling. Exogenous peptide assays and overexpression of nematode CLEs in Arabidopsis demonstrated that CLV2 and CRN are required for perception of nematode CLEs. In addition, promoter–reporter assays showed that both receptors are expressed in nematode-induced syncytia. Lastly, infection assays with receptor mutants revealed a decrease in both nematode infection and syncytium size. Taken together, our results indicate that perception of nematode CLEs by CLV2 and CRN is not only required for successful nematode infection but is also involved in the formation and/or maintenance of nematode-induced syncytia.

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This article is published as Replogle, Amy, Jianying Wang, Andrea Bleckmann, Richard S. Hussey, Thomas J. Baum, Shinichiro Sawa, Eric L. Davis, Xiaohong Wang, Rüdiger Simon, and Melissa G. Mitchum. "Nematode CLE signaling in Arabidopsis requires CLAVATA2 and CORYNE." The Plant Journal 65, no. 3 (2011): 430-440, doi: 10.1111/j.1365-313X.2010.04433.x.

Keywords
CLAVATA2, CORYNE, nematode, CLE peptide, receptor, syncytium
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