Development and Application of Bovine and Porcine Oligonucleotide Arrays with Protein-Based Annotation

dc.contributor.author Garbe, John
dc.contributor.author Elsik, Christine
dc.contributor.author Reecy, James
dc.contributor.author Antoniou, Eric
dc.contributor.author Reecy, James
dc.contributor.author Clark, Karl
dc.contributor.author Venkatraman, Anand
dc.contributor.author Kim, Jae-Woo
dc.contributor.author Schnabel, Robert
dc.contributor.author Dickens, C. Michael
dc.contributor.author Wolfinger, Russell
dc.contributor.author Fahrenkrug, Scott
dc.contributor.author Taylor, Jeremy
dc.contributor.department Animal Science
dc.date 2018-02-17T04:05:32.000
dc.date.accessioned 2020-06-29T23:38:31Z
dc.date.available 2020-06-29T23:38:31Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.issued 2010-01-01
dc.description.abstract <p>The design of oligonucleotide sequences for the detection of gene expression in species with disparate volumes of genome and EST sequence information has been broadly studied. However, a congruous strategy has yet to emerge to allow the design of sensitive and specific gene expression detection probes. This study explores the use of a phylogenomic approach to align transcribed sequences to vertebrate protein sequences for the detection of gene families to design genomewide 70-mer oligonucleotide probe sequences for bovine and porcine. The bovine array contains 23,580 probes that target the transcripts of 16,341 genes, about 72% of the total number of bovine genes. The porcine array contains 19,980 probes targeting 15,204 genes, about 76% of the genes in the Ensembl annotation of the pig genome. An initial experiment using the bovine array demonstrates the specificity and sensitivity of the array.</p>
dc.description.comments <p>This article is from <em>Journal of Biomedicine and Biotechnology</em> 2010 (2010): 453638, doi:<a href="http://dx.doi.org/10.1155/2010/453638" target="_blank">10.1155/2010/453638</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/146/
dc.identifier.articleid 1137
dc.identifier.contextkey 7764660
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/146
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9545
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/146/2010_Reecy_DevelopmentApplication.pdf|||Fri Jan 14 20:23:07 UTC 2022
dc.source.uri 10.1155/2010/453638
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.title Development and Application of Bovine and Porcine Oligonucleotide Arrays with Protein-Based Annotation
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication fb994cd9-94d5-4370-94ab-f33934c4cd6f
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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