Repression of Lignin Biosynthesis Promotes Cellulose Accumulation and Growth in Transgenic Trees
dc.contributor.author | Hu, Wen-Jing | |
dc.contributor.author | Harding, Scott | |
dc.contributor.author | Popko, Jacqueline | |
dc.contributor.author | Ralph, John | |
dc.contributor.author | Stokke, Douglas | |
dc.contributor.author | Tsai, Chung-Jui | |
dc.contributor.author | Chiang, Vincent | |
dc.contributor.department | Forestry | |
dc.date | 2018-02-16T22:58:11.000 | |
dc.date.accessioned | 2020-06-30T03:58:09Z | |
dc.date.available | 2020-06-30T03:58:09Z | |
dc.date.issued | 1999-08-01 | |
dc.description.abstract | <p>Because lignin limits the use of wood for fiber, chemical, and energy production, strategies for its downregulation are of considerable interest. We have produced transgenic aspen (<em>Populus tremuloides</em>Michx.) trees in which expression of a lignin biosynthetic pathway gene<em>Pt4CL1</em> encoding 4-coumarate:coenzyme A ligase (4CL) has been downregulated by antisense inhibition. Trees with suppressed <em>Pt4CL1</em>expression exhibited up to a 45% reduction of lignin, but this was compensated for by a 15% increase in cellulose. As a result, the total lignin–cellulose mass remained essentially unchanged. Leaf, root, and stem growth were substantially enhanced, and structural integrity was maintained both at the cellular and whole-plant levels in the transgenic lines. Our results indicate that lignin and cellulose deposition could be regulated in a compensatory fashion, which may contribute to metabolic flexibility and a growth advantage to sustain the long-term structural integrity of woody perennials.</p> | |
dc.description.comments | <p>This article is from <em>Nature Biotechnology</em> 17 (1999): 808, doi:<a href="http://dx.doi.org/10.1038/11758" target="_blank">10.1038/11758</a>.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/for_pubs/2/ | |
dc.identifier.articleid | 1001 | |
dc.identifier.contextkey | 7528738 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | for_pubs/2 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/37352 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/for_pubs/2/1999_Stokke_RepressionLignin.pdf|||Fri Jan 14 22:05:15 UTC 2022 | |
dc.source.uri | 10.1038/11758 | |
dc.subject.disciplines | Forest Sciences | |
dc.subject.disciplines | Genetics | |
dc.subject.keywords | plant genetic engineering | |
dc.subject.keywords | lignin biosynthesis | |
dc.subject.keywords | 4CLtransgenic | |
dc.subject.keywords | Populus tremuloides | |
dc.title | Repression of Lignin Biosynthesis Promotes Cellulose Accumulation and Growth in Transgenic Trees | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | adc93f91-6757-47de-bf92-f82a880a8aef | |
relation.isOrgUnitOfPublication | e9d5e15e-fc6d-4315-b16b-e7fdff73268a |
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