Association analysis of genes involved in maize (Zea mays L.) root development with seedling and agronomic traits under contrasting nitrogen levels

dc.contributor.author Abdel-Ghani, Adel
dc.contributor.author Kumar, Bharath
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Pace, Jordon
dc.contributor.author Jansen, Constantin
dc.contributor.author Gonzalez-Portilla, Pedro
dc.contributor.author Reyes-Matamoros, Jenaro
dc.contributor.author San Martin, Juan Pablo
dc.contributor.author Lee, Michael
dc.contributor.author Lubberstedt, Thomas
dc.contributor.department Agronomy
dc.date 2018-03-02T14:46:10.000
dc.date.accessioned 2020-06-29T23:05:18Z
dc.date.available 2020-06-29T23:05:18Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.issued 2015-05-01
dc.description.abstract <p>Genotypes with large and well distributed root system might have the potential to adapt to soils with limited nutrient availability. For this purpose, an association study (AS) panel consisting of 74 diverse set of inbred maize lines were screened for seedling root traits and adult plant root traits under two contrasting nitrogen (N) levels (low and high N). Allele re-sequencing of RTCL, RTH3, RUM1, and RUL1 genes related to root development was carried out for AS panel lines. Association analysis was carried out between individual polymorphisms, and both seedling and adult plant traits, while controlling for spurious associations due to population structure and kinship relations. Based on the SNPs identified in RTCL, RTH3, RUM1, and RUL1, lines within the AS panel were grouped into 16, 9, 22, and 7 haplotypes, respectively. Association analysis revealed several polymorphisms within root genes putatively associated with the variability in seedling root and adult plant traits development under contrasting N levels. The highest number of significantly associated SNPs with seedling root traits were found in RTCL (19 SNPs) followed by RUM1 (4 SNPs) and in case of RTH3 and RUL1, two and three SNPs, respectively, were significantly associated with root traits. RTCL and RTH3 were also found to be associated with grain yield. Thus considerable allelic diversity is present within the candidate genes studied and can be utilized to develop functional markers that allow identification of maize lines with improved root architecture and yield under N stress conditions.</p>
dc.description.comments <p>This is a post-peer-review, pre-copyedit version of an article published in Plant Molecular Biology. The final authenticated version is available online at: <a href="http://dx.doi.org/10.1007/s11103-015-0314-1" target="_blank">http://dx.doi.org/10.1007/s11103-015-0314-1</a>.</p> <dl id="x-citeas"><dd id="x-citethis-text"> </dd><dd></dd></dl>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/446/
dc.identifier.articleid 1494
dc.identifier.contextkey 11686263
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/446
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4805
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/446/2015_Lubberstedt_AssociationAnalysis.pdf|||Sat Jan 15 00:19:00 UTC 2022
dc.source.uri 10.1007/s11103-015-0314-1
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Nitrogen
dc.subject.keywords Maize
dc.subject.keywords Root traits
dc.subject.keywords Grain yield
dc.subject.keywords Resequencing
dc.subject.keywords Gene based association mapping
dc.title Association analysis of genes involved in maize (Zea mays L.) root development with seedling and agronomic traits under contrasting nitrogen levels
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 4e4330cd-db15-4ac5-8924-41119139cf32
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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