Enhanced apoptosis as a possible mechanism to self-limit SARSCoV- 2 replication in porcine primary respiratory epithelial cells in contrast to human cells

dc.contributor.author Nelli, Rahul K.
dc.contributor.author Phadke, Kruttika-S
dc.contributor.author Castillo, Gino
dc.contributor.author Yen, Lu
dc.contributor.author Saunders, Amy
dc.contributor.author Rauh, Rolf
dc.contributor.author Nelson, William
dc.contributor.author Bellaire, Bryan H.
dc.contributor.author Giménez-Lirola, Luis
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date.accessioned 2022-04-13T19:53:26Z
dc.date.available 2022-04-13T19:53:26Z
dc.date.issued 2021-12-10
dc.description.abstract The ability of SARS-CoV to infect different species, including humans, dogs, cats, minks, ferrets, hamsters, tigers, and deer, pose a continuous threat to human and animal health. Pigs, though closely related to humans, seem to be less susceptible to SARS-CoV-2. Former in vivo studies failed to demonstrate clinical signs and transmission between pigs, while later attempts using a higher infectious dose reported viral shedding and seroconversion. This study investigated species-specific cell susceptibility, virus dose-dependent infectivity, and infection kinetics, using primary human (HRECs) and porcine (PRECs) respiratory epithelial cells. Despite higher ACE2 expression in HRECs compared to PRECs, SARS-CoV-2 infected, and replicated in both PRECs and HRECs in a dose-dependent manner. Cytopathic effect was particularly more evident in PRECs than HRECs, showing the hallmark morphological signs of apoptosis. Further analysis confirmed an early and enhanced apoptotic mechanism driven through caspase 3/7 activation, limiting SARS-CoV-2 propagation in PRECs compared to HRECs. Our findings shed light on a possible mechanism of resistance of pigs to SARS-CoV-2 infection, and it may hold therapeutic value for the treatment of COVID-19.
dc.description.comments This article is published as Nelli, Rahul K., Kruttika-S. Phadke, Gino Castillo, Lu Yen, Amy Saunders, Rolf Rauh, William Nelson, Bryan H. Bellaire, and Luis G. Giménez-Lirola. "Enhanced apoptosis as a possible mechanism to self-limit SARS-CoV-2 replication in porcine primary respiratory epithelial cells in contrast to human cells." Cell Death Discovery 7, no. 1 (2021): 1-10. DOI: 10.1038/s41420-021-00781-w. Copyright 2021 The Author(s). Attribution 4.0 International (CC BY 4.0). Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/8zn73Xdw
dc.language.iso en
dc.publisher Springer Nature
dc.source.uri https://doi.org/10.1038/s41420-021-00781-w *
dc.title Enhanced apoptosis as a possible mechanism to self-limit SARSCoV- 2 replication in porcine primary respiratory epithelial cells in contrast to human cells
dc.type Article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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