Molecular Mapping of D1, D2 and ms5 Revealed Linkage between the Cotyledon Color Locus D2 and the Male-Sterile Locus ms5 in Soybean

dc.contributor.author Ott, Alina
dc.contributor.author Yang, Yang
dc.contributor.author Bhattacharyya, Madan
dc.contributor.author Horner, Harry
dc.contributor.author Horner, Harry
dc.contributor.author Palmer, Reid
dc.contributor.author Sandhu, Devinder
dc.contributor.department Agronomy
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-17T17:58:33.000
dc.date.accessioned 2020-06-30T04:02:30Z
dc.date.available 2020-06-30T04:02:30Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2013-01-01
dc.description.abstract <p>In soybean, genic male sterility can be utilized as a tool to develop hybrid seed. Several male-sterile, female-fertile mutants have been identified in soybean. The male-sterile, female-fertile ms5 mutant was selected after fast neutron irradiation. Male-sterility due to ms5 was associated with the “stay-green” cotyledon color mutation. The cotyledon color trait in soybean is controlled by two loci, D1 and D2. Association between cotyledon color and male-sterility can be instrumental in early phenotypic selection of sterility for hybrid seed production. The use of such selection methods saves time, money, and space, as fewer seeds need to be planted and screened for sterility. The objectives of this study were to compare anther development between male-fertile and male-sterile plants, to investigate the possible linkages among the Ms5, D1 and D2 loci, and to determine if any of the d1 or d2 mutations can be applied in hybrid seed production. The cytological analysis during anther development displayed optically clear, disintegrating microspores and enlarged, engorged pollen in the male-sterile, female-fertile ms5ms5 plants, a common characteristic of male-sterile mutants. The D1 locus was mapped to molecular linkage group (MLG) D1a and was flanked by Satt408 and BARCSOYSSR_01_1622. The ms5 and D2 loci were mapped to MLG B1 with a genetic distance ~12.8 cM between them. These results suggest that use of the d2 mutant in the selection of male-sterile line may attenuate the cost hybrid seed production in soybean.</p>
dc.description.comments <p>This is article is from <em>Plants </em>2 (2013); 441, doi: <a href="http://dx.doi.org/10.3390/plants2030441" target="_blank">10.3390/plants2030441</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/42/
dc.identifier.articleid 1033
dc.identifier.contextkey 8744218
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/42
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37952
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/42/2013_Horner_MolecularMapping.pdf|||Sat Jan 15 00:12:25 UTC 2022
dc.source.uri 10.3390/plants2030441
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Biology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Glycine max
dc.subject.keywords genetic linkage mapping
dc.subject.keywords cotyledon color
dc.subject.keywords hybrid seed
dc.subject.keywords male sterility
dc.subject.keywords tapetum disintegration
dc.title Molecular Mapping of D1, D2 and ms5 Revealed Linkage between the Cotyledon Color Locus D2 and the Male-Sterile Locus ms5 in Soybean
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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