Microbiome differences in sugarcane and metabolically engineered oilcane accessions and their implications for bioenergy production

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Yang, Jihoon
Sooksa‑nguan, Thanwalee
Kannan, Baskaran
Cano‑Alfanar, Sofia
Liu, Hui
Kent, Angela
Shanklin, John
Altpeter, Fredy
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Springer Nature
Howe, Adina
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Agricultural and Biosystems Engineering

Since 1905, the Department of Agricultural Engineering, now the Department of Agricultural and Biosystems Engineering (ABE), has been a leader in providing engineering solutions to agricultural problems in the United States and the world. The department’s original mission was to mechanize agriculture. That mission has evolved to encompass a global view of the entire food production system–the wise management of natural resources in the production, processing, storage, handling, and use of food fiber and other biological products.

In 1905 Agricultural Engineering was recognized as a subdivision of the Department of Agronomy, and in 1907 it was recognized as a unique department. It was renamed the Department of Agricultural and Biosystems Engineering in 1990. The department merged with the Department of Industrial Education and Technology in 2004.

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  • Department of Agricultural Engineering (1907–1990)

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Oilcane is a metabolically engineered sugarcane (Saccharum spp. hybrid) that hyper-accumulates lipids in its vegetable biomass to provide an advanced feedstock for biodiesel production. The potential impact of hyper-accumulation of lipids in vegetable biomass on microbiomes and the consequences of altered microbiomes on plant growth and lipid accumulation have not been explored so far. Here, we explore differences in the microbiome structure of different oilcane accessions and non-modified sugarcane. 16S SSU rRNA and ITS rRNA amplicon sequencing were performed to compare the characteristics of the microbiome structure from different plant compartments (leaf, stem, root, rhizosphere, and bulk soil) of four greenhouse-grown oilcane accessions and non-modified sugarcane. Significant differences were only observed in the bacterial microbiomes. In leaf and stem microbiomes, more than 90% of the entire microbiome of non-modified sugarcane and oilcane was dominated by similar core taxa. Taxa associated with Proteobacteria led to differences in the non-modified sugarcane and oilcane microbiome structure. While differences were observed between multiple accessions, accession 1566 was notable in that it was consistently observed to differ in its microbial membership than other accessions and had the lowest abundance of taxa associated with plant-growth-promoting bacteria. Accession 1566 is also unique among oilcane accessions in that it has the highest constitutive expression of the WRI1 transgene. The WRI1 transcription factor is known to contribute to significant changes in the global gene expression profile, impacting plant fatty acid biosynthesis and photomorphogenesis. This study reveals for the first time that genetically modified oilcanes associate with distinct microbiomes. Our findings suggest potential relationships between core taxa, biomass yield, and TAG in oilcane accessions and support further research on the relationship between plant genotypes and their microbiomes.
This article is published as Yang, Jihoon, Thanwalee Sooksa-Nguan, Baskaran Kannan, Sofia Cano-Alfanar, Hui Liu, Angela Kent, John Shanklin, Fredy Altpeter, and Adina Howe. "Microbiome differences in sugarcane and metabolically engineered oilcane accessions and their implications for bioenergy production." Biotechnology for Biofuels and Bioproducts 16, no. 1 (2023): 1-14. DOI: 10.1186/s13068-023-02302-6. Copyright 2023 The Author(s). Attribution 4.0 International (CC BY 4.0). Posted with permission.