Separation and Identification of Highly Efficient Antioxidant Peptides from Eggshell Membrane

dc.contributor.author Zhao, Qian-Cheng
dc.contributor.author Zhao, Jie-Yuan
dc.contributor.author Ahn, Dong
dc.contributor.author Ahn, Dong Uk
dc.contributor.author Jin, Yong-Guo
dc.contributor.author Huang, Xi
dc.contributor.department Animal Science
dc.date 2021-05-25T20:56:46.000
dc.date.accessioned 2021-08-14T02:08:42Z
dc.date.available 2021-08-14T02:08:42Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-01-01
dc.description.abstract <p>The enzymatic hydrolysates (EHs) of the eggshell membrane (ESM) were obtained after incubating eggshell membrane in solutions prepared with Na<sub>2</sub>SO<sub>3</sub> and alkaline protease combinations. The effects of enzyme species, enzyme dosage, Na<sub>2</sub>SO<sub>3</sub> concentration, and hydrolysis time on the antioxidant activity of the ESM-EH were determined. Also, the correlation between the degree of hydrolysis (DH) and the antioxidant activity of ESM-EH was analyzed. The DH of ESM-EH showed a highly positive correlation with the reducing power (R<sup>2</sup> = 0.857) and total antioxidant activity (TAA) (R<sup>2</sup> = 0.876) and performed negative correlation with the Fe<sup>2+</sup>-chelating ability (R<sup>2</sup> = −0.529). The molecular weight distribution of the ESM-EH was determined by MALDI-TOF/MS. Cation exchange chromatography (Sephadex C-25) was used to isolate the ESM-EH and then the enzymatic hydrolysis fragment (EHF) was obtained. Among the five isolated fragments (F1~F5), fragment 3 (F3), which was composed of 28 polypeptides, showed the highest ability to quench ABTS• (2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid) (90.44%) and also displayed stronger TBARS (thiobarbituric acid– reactive substances) (58.17%) and TAA (303.82 µg /mL) than the ESM-EH. Further analysis of the 28 peptides in F3 identified using LC-MS/MS indicated that five peptides (ESYHLPR, NVIDPPIYAR, MFAEWQPR, LLFAMTKPK, MLKMLPFK) showed high water-solubility, biological activities, and antioxidant characteristics. Finally, the TAA of the synthetic peptide was verified, the synthetic peptides ESYHLPR and MFAEWQPR performed the best activity and have high potentials to be used as antioxidant agents in functional foods, pharmaceuticals, or cosmetics.</p>
dc.description.comments <p>This article is published as Zhao, Qian-Cheng, Jie-Yuan Zhao, Dong Uk Ahn, Yong-Guo Jin, and Xi Huang. "Separation and identification of highly efficient antioxidant peptides from eggshell membrane." <em>Antioxidants</em> 8, no. 10 (2019): 495. doi:<a href="https://doi.org/10.3390/antiox8100495">10.3390/antiox8100495</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/867/
dc.identifier.articleid 1863
dc.identifier.contextkey 23080810
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/867
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/6wBlnjar
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/867/0-Supplementary_File_1.pdf|||Sat Jan 15 02:14:56 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/867/2019_Ahn_SeparationIdentification.pdf|||Sat Jan 15 02:14:57 UTC 2022
dc.source.uri 10.3390/antiox8100495
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords eggshell membrane
dc.subject.keywords Na2SO3-assisted enzymatic hydrolysis
dc.subject.keywords antioxidant peptide
dc.subject.keywords inhibition of liposome peroxidation
dc.subject.keywords mass spectrometry
dc.subject.keywords total antioxidant activity
dc.title Separation and Identification of Highly Efficient Antioxidant Peptides from Eggshell Membrane
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication d3386101-2f0d-4375-ab44-ac1addb6a9ad
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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