Spatial Mapping and Profiling of Metabolite Distributions During Germination Nikolau, Basil Yandeau-Nelson, Marna Feenstra, Adam Lee, Young Jin Alexander, Liza Song, Zhihong Korte, Andrew Yandeau-Nelson, Marna Nikolau, Basil Lee, Young-Jin
dc.contributor.department Chemistry
dc.contributor.department Ames Laboratory
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Ames Laboratory 2018-06-29T21:21:07.000 2020-06-30T01:15:59Z 2020-06-30T01:15:59Z Sun Jan 01 00:00:00 UTC 2017 2017-08-01
dc.description.abstract <p>Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also revealed that many compounds were highly sequestered among the various seed tissue types. To further localize compounds, matrix-assisted laser desorption/ionization MSI is utilized to visualize compounds in fine detail in their native environments over the course of germination. Most notably, the fatty acyl chain-dependent differential localization of phospholipids and TAGs were observed within the embryo and radicle, showing correlation with the heterogeneous distribution of fatty acids. Other interesting observations include unusual localization of ceramides on the endosperm/scutellum boundary, and subcellular localization of ferulate in the aleurone.</p>
dc.description.comments <p>This article is published as Feenstra, Adam D., Liza E. Alexander, Zhihong Song, Andrew R. Korte, Marna Yandeau-Nelson, Basil J. Nikolau, and Young-Jin Lee. "Spatial mapping and profiling of metabolite distributions during germination." <em>Plant Physiology</em> (2017): 2532. DOI: <a href="" target="_blank">10.1104/pp.17.00652</a> Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 2017
dc.identifier.contextkey 11021011
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/1016
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:14:58 UTC 2022
dc.source.uri 10.1104/pp.17.00652
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Organic Chemistry
dc.subject.disciplines Plant Biology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords amino acids
dc.subject.keywords B73
dc.subject.keywords ceramides
dc.subject.keywords ferulate
dc.subject.keywords germination
dc.subject.keywords maize
dc.subject.keywords mass spectrometry imaging
dc.subject.keywords metabolomics
dc.subject.keywords Mo17
dc.subject.keywords phospholipids
dc.title Spatial Mapping and Profiling of Metabolite Distributions During Germination
dc.type article
dc.type.genre article
dspace.entity.type Publication
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