Fibroblasts treated with macrophage conditioned medium results in phenotypic shifts and changes in collagen organization

dc.contributor.author Li, Zhuqing
dc.contributor.author Bratlie, Kaitlin
dc.contributor.author Bratlie, Kaitlin
dc.contributor.department Materials Science and Engineering
dc.contributor.department Chemical and Biological Engineering
dc.date 2021-02-03T17:44:32.000
dc.date.accessioned 2021-02-26T03:30:07Z
dc.date.available 2021-02-26T03:30:07Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2021
dc.date.embargo 2023-01-29
dc.date.issued 2021-01-29
dc.description.abstract <p>In tissue regeneration, the goal is to regenerate tissue similar to what was damaged or missing while preventing fibrotic scarring, which may lead to decreased mechanical strength and dissimilar tissue characteristics compared to native tissue. We believe collagen orientation plays a critical role in wound contraction and scarring and that it is modulated by myofibroblasts. We used macrophage conditioned medium to simulate complex events that can influence the fibroblast phenotype during the wound healing process. In addition to examining the effect of macrophage phenotype on fibroblasts, we inhibited focal adhesion kinase (FAK), Rho-associated protein kinase (ROCK), and myosin II for fibroblasts cultured on both tissue culture plastic and methacrylated gellan gum to understand how different pathways and materials influence fibroblast responses. Collagen orientation, α-SMA expression, focal adhesion area, and cell migration were altered by inhibition of FAK, ROCK, or myosin II and macrophage phenotype, along with the substrate. An increase in either focal adhesion area or α-smooth muscle actin (α-SMA) expression correlated with an aligned collagen orientation. Gellan gum hydrogel upregulated α-SMA expression in ROCK inhibited conditioned media and downregulated the FAK area in FAK and ROCK inhibited conditioned media. Myosin II had no impact on the α-SMA expression on the substrate compared to coverslip except for M2 conditioned medium. Gellan gum hydrogel significantly increased cell migration under FAK and Myosin II mediated conditioned media and unconditioned media. Collectively, our study examined how macrophage phenotype influences fibroblast response, which would be beneficial in controlling scar tissue formation.</p>
dc.description.comments <p>This is a manuscript of an article published as Li, Zhuqing, and Kaitlin M. Bratlie. "Fibroblasts treated with macrophage conditioned medium results in phenotypic shifts and changes in collagen organization." <em>Materials Science and Engineering: C</em> (2021): 111915. DOI: <a href="https://doi.org/10.1016/j.msec.2021.111915" target="_blank">10.1016/j.msec.2021.111915</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/mse_pubs/398/
dc.identifier.articleid 1401
dc.identifier.contextkey 21443538
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath mse_pubs/398
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/96729
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/mse_pubs/398/2021_BratlieKatilin_FibroblastsTreated.pdf|||Fri Jan 14 23:56:46 UTC 2022
dc.source.uri 10.1016/j.msec.2021.111915
dc.subject.disciplines Biology and Biomimetic Materials
dc.subject.disciplines Cell Biology
dc.subject.keywords Wound healing
dc.subject.keywords Fibrosis
dc.subject.keywords Collagen alignment
dc.subject.keywords Cell pathway inhibitors
dc.subject.keywords Methacrylated gellan gum
dc.subject.keywords Cell migration
dc.title Fibroblasts treated with macrophage conditioned medium results in phenotypic shifts and changes in collagen organization
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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