Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection

Thumbnail Image
Date
2009-03-01
Authors
Bisha, Bledar
Brehm-Stecher, Byron
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Research Projects
Organizational Units
Organizational Unit
Journal Issue
Is Version Of
Versions
Series
Department
Food Science and Human Nutrition
Abstract

A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.

Comments

This article is from Applied and Environmental Microbiology 75, no. 5 (March 2009): 1450–1455, doi:10.1128/AEM.01944-08.

Description
Keywords
Citation
DOI
Copyright
Thu Jan 01 00:00:00 UTC 2009
Collections