Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection

Date
2009-03-01
Authors
Bisha, Bledar
Brehm-Stecher, Byron
Brehm-Stecher, Byron
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Journal Issue
Series
Department
Food Science and Human Nutrition
Abstract

A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.

Comments

This article is from Applied and Environmental Microbiology 75, no. 5 (March 2009): 1450–1455, doi:10.1128/AEM.01944-08.

Description
Keywords
Citation
DOI
Collections