Systems analyses of key metabolic modules of floral and extrafloral nectaries of cotton

Thumbnail Image
Date
2019-11-27
Authors
Chatt, Elizabeth
Mahalim, Siti-Nabilla
Mohd-Fadzil, Nur-Aziatull
Roy, Rahul
Klinkenberg, Peter
Hampton, Marshall
Carter, Clay
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Person
Horner, Harry
University Professor Emeritus
Person
Nikolau, Basil
Emeritus Faculty
Research Projects
Organizational Units
Organizational Unit
Genetics, Development and Cell Biology

The Department of Genetics, Development, and Cell Biology seeks to teach subcellular and cellular processes, genome dynamics, cell structure and function, and molecular mechanisms of development, in so doing offering a Major in Biology and a Major in Genetics.

History
The Department of Genetics, Development, and Cell Biology was founded in 2005.

Related Units

Journal Issue
Is Version Of
Versions
Series
Department
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
Abstract

Nectar is a primary reward mediating plant-animal mutualisms to improve plant fitness and reproductive success. In Gossypium hirsutum (cotton), four distinct trichomatic nectaries develop, one floral and three extrafloral. The secreted floral and extrafloral nectars serve different purposes, with the floral nectar attracting bees to promote pollination and the extrafloral nectar attracting predatory insects as a means of indirect resistance from herbivores. Cotton therefore provides an ideal system to contrast mechanisms of nectar production and nectar composition between floral and extrafloral nectaries. Here, we report the transcriptome, ultrastructure, and metabolite spatial distribution using mass spectrometric imaging of the four cotton nectary types throughout development. Additionally, the secreted nectar metabolomes were defined and were jointly composed of 197 analytes, 60 of which were identified. Integration of theses datasets support the coordination of merocrine-based and eccrine-based models of nectar synthesis. The nectary ultrastructure supports the merocrine-based model due to the abundance of rough endoplasmic reticulum positioned parallel to the cell walls and profusion of vesicles fusing to the plasma membranes. The eccrine-based model which consist of a progression from starch synthesis to starch degradation and to sucrose biosynthesis was supported by gene expression data. This demonstrates conservation of the eccrine-based model for the first time in both trichomatic and extrafloral nectaries. Lastly, nectary gene expression data provided evidence to support de novo synthesis of amino acids detected in the secreted nectars.

Comments

This preprint of an article published as Chatt, EC, S-N Mahalim, N-A Mohd-Fadzil, Rl Roy, PM Klinkenberg, HTHorner, M Hampton, CJ Carter, BJ Nikolau. 2021. Systems analyses of key metabolic modules of floral and extrafloral nectaries of cotton (Gossypium hirsutum). Plant Physiology 185: 1595-1616 10.1101/857771. Posted with permission.

Description
Keywords
Citation
DOI
Copyright
Fri Jan 01 00:00:00 UTC 2021
Collections