Microtissue size and cell-cell communication modulate cell migration in arrayed 3D collagen gels

dc.contributor.author Che, Xiangchen
dc.contributor.author Nuhn, Jacob
dc.contributor.author Gong, Shenmin
dc.contributor.author Che, Xiangchen
dc.contributor.author Schneider, Ian
dc.contributor.author Que, Long
dc.contributor.author Schneider, Ian
dc.contributor.department Electrical and Computer Engineering
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemical and Biological Engineering
dc.date 2018-10-10T09:35:23.000
dc.date.accessioned 2020-06-30T01:09:49Z
dc.date.available 2020-06-30T01:09:49Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2018
dc.date.embargo 2019-07-30
dc.date.issued 2018-09-01
dc.description.abstract <p>Cells communicate through the extracellular matrix (ECM) in many physiological and pathological processes. This is particularly important during cell migration, where cell communication can alter both the speed and the direction of migration. However, most cell culture systems operate with large volumes relative to cell numbers, creating low cell densities and diluting factors that mediate cell communication. Furthermore, they lack the ability to isolate single cells or small groups of cells. Droplet forming devices allow for an ability to embed single or small groups of cells into small volume segregated 3D environments, increasing the cell density to physiological levels. In this paper we show a microfluidic droplet device for fabricating 3D collagen-based microtissues to study breast cancer cell motility. MDA-MB-231 cells fail to spread and divide in small, thin chambers. Cell migration is also stunted as compared to thick 3D gels. However, larger chambers formed by a thicker devices promote cell spreading, cell division and faster migration. In the large devices, both cell-ECM and cell-cell interactions affect cell motility. Increasing collagen density decreases cell migration and increasing the number of cells per chamber increases cell migration speed. Furthermore, cells appear to sense both the ECM-chamber wall interface as well as other cells. Cells migrate towards the ECM-chamber interface if within roughly 150 μm, whereas cells further than 150 μm tend to move towards the center of the chamber. Finally, while cells do not show enhanced movement towards the center of mass of a cell cluster, their migration speed is more variable when further away from the cell cluster center of mass. These results show that microfluidic droplet devices can array 3D collagen gels and promote cell spreading, division and migration similar to what is seen in thick 3D collagen gels. Furthermore, they can provide a new avenue to study cell migration and cell-cell communication at physiologically relevant cell densities.</p>
dc.description.comments <p>This is a post-peer-review, pre-copyedit version of an article published in <em>Biomedical Microdevices</em>. The final authenticated version is available online at: <a href="https://doi.org/10.1007/s10544-018-0309-1" target="_blank">https://doi.org/10.1007/s10544-018-0309-1</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/345/
dc.identifier.articleid 1347
dc.identifier.contextkey 13052287
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/345
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13446
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/345/2018_Schneider_MicrotissueSize.pdf|||Fri Jan 14 23:42:20 UTC 2022
dc.source.uri 10.1007/s10544-018-0309-1
dc.subject.disciplines Biomedical Devices and Instrumentation
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Electrical and Computer Engineering
dc.subject.keywords Paracrine
dc.subject.keywords Autocrine
dc.subject.keywords Cell-cell communication
dc.subject.keywords Soft-stiff interfaces
dc.subject.keywords Stiffness
dc.subject.keywords Motility
dc.title Microtissue size and cell-cell communication modulate cell migration in arrayed 3D collagen gels
dc.type article
dc.type.genre article
dspace.entity.type Publication
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