Genetic Diversity and Antimicrobial Susceptibility of Campylobacter jejuni Isolates Associated with Sheep Abortion in the United States and Great Britain

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dc.contributor.author Plummer, Paul
dc.contributor.author Wu, Zuowei
dc.contributor.author Sippy, Rachel
dc.contributor.author Sahin, Orhan
dc.contributor.author Plummer, Paul
dc.contributor.author Zhang, Qijing
dc.contributor.author Vidal, Ana
dc.contributor.author Newell, Diane
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-02-17T10:08:30.000
dc.date.accessioned 2020-07-07T05:13:22Z
dc.date.available 2020-07-07T05:13:22Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-06-01
dc.description.abstract <p>Campylobacter infection is a leading cause of ovine abortion worldwide. Historically, genetically diverse Campylobacter fetus and Campylobacter jejunistrains have been implicated in such infections, but since 2003 a highly pathogenic, tetracycline-resistant C. jejuni clone (named SA) has become the predominant cause of sheep abortions in the United States. Whether clone SA was present in earlier U.S. abortion isolates (before 2000) and is associated with sheep abortions outside the United States are unknown. Here, we analyzed 54 C. jejuniisolates collected from U.S. sheep abortions at different time periods and compared them with 42 C. jejuni isolates associated with sheep abortion during 2002 to 2008 in Great Britain, using multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and array-based comparative genomic hybridization (CGH). Although clone SA (ST-8) was present in the early U.S. isolates, it was not as tetracycline resistant (19% versus 100%) or predominant (66% versus 91%) as it was in the late U.S isolates. In contrast, C. jejuni isolates from Great Britain were genetically diverse, comprising 19 STs and lacking ST-8. PFGE and CGH analyses of representative strains further confirmed the population structure of the abortion isolates. Notably, the Great Britain isolates were essentially susceptible to most tested antibiotics, including tetracycline, while the late U.S. isolates were universally resistant to this antibiotic, which could be explained by the common use of tetracyclines for control of sheep abortions in the United States but not in Great Britain. These results suggest that the dominance of clone SA in sheep abortions is unique to the United States, and the use of tetracyclines may have facilitated selection of this highly pathogenic clone.</p>
dc.description.comments <p>This article is from <em>Journal of Clinical Microbiology</em> 52 (2014): 1853, doi:<a href="http://dx.doi.org/10.1128/JCM.00355-14" target="_blank">10.1128/JCM.00355-14</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/39/
dc.identifier.articleid 1038
dc.identifier.contextkey 7988190
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/39
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92065
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/39/2014_Plummer_GeneticDiversity.pdf|||Fri Jan 14 23:54:48 UTC 2022
dc.source.uri 10.1128/JCM.00355-14
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Genetic Diversity and Antimicrobial Susceptibility of Campylobacter jejuni Isolates Associated with Sheep Abortion in the United States and Great Britain
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication f16d5130-7036-437d-892d-b4a4072903e7
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