The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization Raphael, Brian Pereira, Sonia Flom, Gary Zhang, Qijing Ketley, Julian Konkel, Michael
dc.contributor.department Veterinary Microbiology and Preventive Medicine 2018-02-18T23:27:43.000 2020-07-07T05:14:46Z 2020-07-07T05:14:46Z Sat Jan 01 00:00:00 UTC 2005 2005-06-01
dc.description.abstract <p>Two-component regulatory systems play a major role in the physiological response of bacteria to environmental stimuli. Such systems are composed of a sensor histidine kinase and a response regulator whose ultimate function is to affect the expression of target genes. Response regulator mutants of <em>Campylobacter jejuni</em> strain F38011 were screened for sensitivity to sodium deoxycholate. A mutation in Cj0643, which encodes a response regulator with no obvious cognate histidine kinase, resulted in an absence of growth on plates containing a subinhibitory concentration of sodium deoxcholate (1%, wt/vol). In broth cultures containing 0.05% (wt/vol) sodium deoxycholate, growth of the mutant was significantly inhibited compared to growth of the <em>C. jejuni</em> F38011 wild-type strain. Complementation of the <em>C. jejuni cbrR</em> mutant in <em>trans</em> restored growth in both broth and plate cultures supplemented with sodium deoxycholate. Based on the phenotype displayed by its mutation, we designated the gene corresponding to Cj0643 as <em>cbrR</em> (<em>Campylobacter b</em>ile <em>r</em>esistance <em>r</em>egulator). While the MICs of a variety of bile salts and other detergents for the <em>C. jejuni cbrR</em> mutant were lower, no difference was noted in its sensitivity to antibiotics or osmolarity. Finally, chicken colonization studies demonstrated that the <em>C. jejuni cbrR</em> mutant had a reduced ability to colonize compared to the wild-type strain. These data support previous findings that bile resistance contributes to colonization of chickens and establish that the response regulator, CbrR, modulates resistance to bile salts in <em>C. jejuni</em>.</p>
dc.description.comments <p>This article is published as Raphael, Brian H., Sonia Pereira, Gary A. Flom, Qijing Zhang, Julian M. Ketley, and Michael E. Konkel. "The Campylobacter jejuni response regulator, CbrR, modulates sodium deoxycholate resistance and chicken colonization." Journal of bacteriology 187, no. 11 (2005): 3662-3670. doi: <a href="" target="_blank" title=" The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization ">10.1128/JB.187.11.3662-3670.2005</a>. Posted with permission.</p>
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dc.identifier.articleid 1171
dc.identifier.contextkey 10837281
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dc.identifier.submissionpath vmpm_pubs/171
dc.language.iso en
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dc.source.uri 10.1128/JB.187.11.3662-3670.2005
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Physiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization
dc.type article
dc.type.genre article
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relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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