Does Alternative Transposition, a Potential Genome-restructuring Activity, Occur in Petunia?

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dc.contributor.author Gilbert, Kaitlyn
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-14T09:57:44.000
dc.date.accessioned 2020-07-07T05:10:26Z
dc.date.available 2020-07-07T05:10:26Z
dc.date.issued 2014-04-15
dc.description.abstract <p>Transposable Elements (TEs) are segments of DNA that can move throughout the genome. TEs are present in most species and can cause insertions and deletions in the genetic sequence. TEs encode a transposase enzyme which excises the TE and inserts it elsewhere. This is considered standard transposition. Alternative Transposition occurs when transposase acts on the termini of two different TEs. This action can result in major chromosomal rearrangements or chromosomal breakage. Alternative Transposition has been observed previously with maize Ac/Ds elements; here, we are asking whether it also occurs in Petunia hybrida. Petunias have dTph1 TEs which are small, non-autonomous transposons. The S857 allele contains two copies of dTph1 located approximately 30 bp apart and facing in opposite orientations. PCR (Polymerase Chain Reaction) will be used to identify petunias with both elements, and PCR experiments with primers facing in the same direction will be used to test for Alternative Transposition. A product should be generated only if Alternative Transposition has occurred and the primers are re-oriented to face one another. The results may provide evidence that Alternative Transposition can occur in multiple species, suggesting that it may have had a significant impact on the evolution of plant genomes.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/23/
dc.identifier.articleid 1022
dc.identifier.contextkey 5914582
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2014/presentations/23
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91585
dc.relation.ispartofseries Symposium on Undergraduate Research and Creative Expression
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/23/GilbertKaitlyn.pdf|||Fri Jan 14 22:45:35 UTC 2022
dc.subject.disciplines Biochemistry
dc.subject.disciplines Genomics
dc.title Does Alternative Transposition, a Potential Genome-restructuring Activity, Occur in Petunia?
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isSeriesOfPublication 6730f354-97b8-4408-bad3-7e5c3b2fca9d
thesis.degree.discipline Biology
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