Considerations in the use of processing fluids for the detection of PRRSV RNA and antibody
Date
2022-08-10
Authors
López, Will
Zimmerman, Jeff
Gauger, Phil
Harmon, Karen
Magtoto, Ronaldo
Bradner, Laura
Holtkamp, Derald
Zhang, Min
Zhang, Jianqiang
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Sage Journals
Abstract
Surveillance is mandatory for tracking the progress of porcine reproductive and respiratory syndrome virus (PRRSV) control and elimination efforts in breeding herds. Processing fluids, the fluid recovered from tissues collected at castration and/or tail docking, are used for breeding herd surveillance by large segments of the industry, but the basic diagnostic characteristics of processing fluids are largely undescribed. We undertook 3 studies to address this information gap. In study 1, we found no differences among the PRRSV RT-rtPCR results obtained with 4 commercial RNA extraction kits. In study 2, we found that PRRSV RNA was highly stable in processing fluid samples at −20°C or 4°C, but detrimental effects were observed at ≥22°C within 24 h. In study 3, using a modified PRRSV ELISA at a sample:positive cutoff of ≥0.5, we found excellent discrimination in the detection of PRRSV antibody (IgM, IgA, IgG) in processing fluids from herds of known PRRSV status. Judicious handling of processing fluid samples from sow herds, and the use of methods available in veterinary diagnostic laboratories, can provide a foundation for reliable PRRSV surveillance.
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Comments
This is a manuscript of an article published as López, Will, Jeff Zimmerman, Phil Gauger, Karen Harmon, Ronaldo Magtoto, Laura Bradner, Derald Holtkamp et al. "Considerations in the use of processing fluids for the detection of PRRSV RNA and antibody." Journal of Veterinary Diagnostic Investigation 34, no. 5 (2022): 859-863. doi: https://doi.org/10.1177/10406387221114855. Posted with Permission. Copyright The Authors 2022.