Separation of Ovotransferrin from Chicken Egg White without Using Organic Solvents Abeyrathne, Sandun Lee, Hyunyong Ahn, Dong Ahn, Dong 2018-08-25T22:19:06.000 2020-06-29T23:35:53Z 2020-06-29T23:35:53Z Thu Jan 01 00:00:00 UTC 2015 2015-01-01
dc.description.abstract <p>Ovotransferrin from chicken egg white was separated without using organic solvents. Egg white was diluted with 1 vol. of distilled water (DW), and then homogenized. After removing ovomucin by centrifugation after adjusting the pH to 4.5-5.0, the resulting supernatant was added with ammonium sulfate and citric acid, and then centrifuged after holding overnight at 4 oC. The precipitant, which contains ovotransferrin, was dissolved in DW and the ovotransferrin was re-precipitated using ammonium sulfate and citric acid. The precipitant collected after centrifugation was dissolved with DW, and then desalted and concentrated. The purity of ovotransferrin was determined, the protein identified, and the yield calculated after freeze drying. Over 85% purity and over 83% yield were obtained from the combinations of 5.0% (w/v) ammonium sulfate and 2.5% (w/v) citric acid followed by 2.0% (w/v) ammonium sulfate and 1.5% (w/v) citric acid. The method was simple and cost effective. The isolated ovotransferrin can be used as is or after modifications for various applications such as antimicrobial, anticancer treatments, and iron supplementing agents for human.</p>
dc.identifier archive/
dc.identifier.articleid 2099
dc.identifier.contextkey 6761509
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_air/vol661/iss1/53
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R2990
dc.source.bitstream archive/|||Sat Jan 15 00:49:57 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords ASL R2990
dc.subject.keywords Animal Science
dc.title Separation of Ovotransferrin from Chicken Egg White without Using Organic Solvents
dc.type article
dc.type.genre poultry
dspace.entity.type Publication
relation.isAuthorOfPublication d3386101-2f0d-4375-ab44-ac1addb6a9ad
relation.isJournalIssueOfPublication 6758d906-7d2a-4198-98dc-f5f25ab96561
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
108.32 KB
Adobe Portable Document Format