Effect of temperature and relative humidity on the stability ofinfectious porcine reproductive and respiratory syndrome virus in aerosols

Date
2007-01-01
Authors
Hermann, Joseph
Hoff, Steven
Hoff, Steven
Munoz-Zanzi, Claudia
Yoon, Kyoung-Jin
Roof, Micahel
Burkhardt, Anna
Zimmerman, Jeffrey
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Agricultural and Biosystems Engineering
Abstract

The objective of this experiment was to describe the stability of airborne infectious porcine reproductive and respiratory syndrome virus (PRRSV) as a function of temperature and relative humidity. A cloud of infectious PRRSV was aerosolized using 24-jet Collison nebulizer into a dynamic aerosoltoroid (DAT) maintained at a specific temperature and relative humidity. The PRRSV cloud within the DAT was sampled repeatedly over time using SKC BioSampler impingers and the total viral RNA (RT-PCR) and concentration of infectious PRRSV (TCID in the air samples was determined. As measured by quantitative RT-PCR, PRRSV RNA was stable under the conditions evaluated in this study. Thus, a comparison of viral RNA and Rhodamine B dye, a physical tracer, found no significant difference in the slopes of the lines. Titers of infectious virus were plotted by time and the half-life (1/2) of infectious PRRSV was calculated using linear regression analysis. An analysis of the results showed that aerosolized PRRSV was more stable at lower temperatures and/or lower relative humidity, but temperature had a greater effect on the 1/2 of PRRSV than relative humidity. Based on these results, an equation was derived to predict the 1/2 of infectious airborne PRRSV for any combination of environmental temperature and relative humidity.

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This article is from Veterinary Research 38, no. 1 (2007): 81–93, doi:10.1051/vetres:2006044. Posted with permission.

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