Rapid Communication: Restriction Fragment Length Polymorphisms at the Porcine Transporter Associated with Antigen Processing 1 (TAP1) Locus
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1994
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Abstract
Source and Description of Probe. A 2.7-kb human cDNA clone for TAP1 was excised from the XbaI site of the plasmid pRSV.5neo (Spies et al., 1990). Method of Detection. Hybridizations were performed at 65°C for 16 to 20 h (10% dextran sulfate, 7% SDS, .263 M NazHP04, 1% BSA, 1 mM EDTA, 100 pg/mL sonicated denatured salmon sperm DNA). Final washes were done at 65°C in .7x SSC, .5% SDS for 15 to 20 min.
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This is an article from Journal of Animal Science 72 (1994): 255, doi:/1994.721255x. Posted with permission.
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Sat Jan 01 00:00:00 UTC 1994