Identification of a nth-Like Gene Encoding an Endonuclease III in Campylobacter jejuni Dai, Lei Xia, Jing Sahin, Orhan Zhang, Qijing Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine 2019-05-27T10:47:32.000 2020-07-07T05:15:01Z 2020-07-07T05:15:01Z Tue Jan 01 00:00:00 UTC 2019 2019-04-10
dc.description.abstract <p><em>Campylobacter jejuni</em> is a leading cause of foodborne illnesses worldwide. As a microaerobic pathogen, <em>C. jejuni</em> is subjected to DNA damages caused by various stresses such as reactive oxygen species (ROS) and UV radiations. The base excision repair (BER) system plays an important role in preventing mutations associated with oxidative DNA damage, but the system remains poorly characterized in <em>Campylobacter</em>. In this study, a BER homolog encoded by <em>cj0595c</em> (named <em>nth</em>) in <em>C. jejuni</em> was analyzed for endonuclease III activity and for its role in maintaining genomic stability. It was found that inactivation of <em>nth</em> resulted in elevated frequencies of spontaneous fluoroquinolone-resistant (FQR) and oxidative stress resistant (OXR) mutants, compared with the wild-type strain in <em>C. jejuni</em>. Sequencing analysis of the FQR and OXR mutants revealed that the elevated mutation rates were associated with C → T or G → A transition in <em>gyrA</em> (FQR mutants) <em>or perR</em> (for OXR mutants). In an <em>in vitro</em> assay, a purified recombinant <em>C. jejuni</em> Nth protein demonstrated endonuclease III activity that recognized and excised the thymine glycol (Tg) base from a double stranded DNA. These findings indicate that Nth functions as a BER repair enzyme in <em>C. jejuni</em> and is important for the repair of DNA damage, protecting the bacteria from stresses encountered within a host and in the environment.</p>
dc.description.comments <p>This article is published as Dai, Lei, Orhan Sahin, and Qijing Zhang. "Identification of a nth‐like Gene encoding an Endonuclease III in Campylobacter jejuni." <em>Frontiers in Microbiology</em> 10 (2019): 698. DOI: <a href="" target="_blank">10.3389/fmicb.2019.00698</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1208
dc.identifier.contextkey 14222927
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/209
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 22:30:08 UTC 2022
dc.source.uri 10.3389/fmicb.2019.00698
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Identification of a nth-Like Gene Encoding an Endonuclease III in Campylobacter jejuni
dc.type article
dc.type.genre article
dspace.entity.type Publication
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