Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy

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dc.contributor.author Anderson, Timothy
dc.contributor.author Ai, Yongfeng
dc.contributor.author Birt, Diane
dc.contributor.author Jones, Roger
dc.contributor.author Jane, Jay-Lin
dc.contributor.author Houk, Robert
dc.contributor.author Zhao, Yinsheng
dc.contributor.author McClelland, John
dc.contributor.department Ames National Laboratory
dc.date 2018-02-14T10:35:42.000
dc.date.accessioned 2020-06-29T23:25:29Z
dc.date.available 2020-06-29T23:25:29Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.embargo 2014-08-21
dc.date.issued 2013-02-27
dc.description.abstract <p>Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. To calibrate the FTIR-PAS analysis, samples from each diet were analyzed using an enzymatic assay. A partial least-squares cross-validation plot generated from the enzymatic assay and FTIR-PAS spectral results for starch fit the ideal curve with a <em>R</em>2 of 0.997. A principal component analysis plot of components 1 and 2 showed that spectra from diets clustered significantly from each other. This study clearly showed that FTIR-PAS can accurately quantify starch content and identify the form of starch in complex matrices.</p>
dc.description.comments <p>Reprinted with permission from <em>Journal of Agricultural and Food Chemistry</em> 61 (2013): 1818–1822, doi:10.1021/jf3042616. Copyright 2013 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ameslab_pubs/155/
dc.identifier.articleid 1155
dc.identifier.contextkey 6015752
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ameslab_pubs/155
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/7698
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ameslab_pubs/155/0-2012_Anderson_AnalysisResistant_License.pdf|||Fri Jan 14 20:41:52 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/ameslab_pubs/155/2013_Anderson_AnalysisResistant.pdf|||Fri Jan 14 20:41:54 UTC 2022
dc.source.uri 10.1021/jf3042616
dc.subject.disciplines Acoustics, Dynamics, and Controls
dc.subject.disciplines Food Chemistry
dc.subject.disciplines Organic Chemistry
dc.subject.keywords Biochemistry Biophysics and Molecular Biology
dc.subject.keywords Chemistry
dc.subject.keywords Food Science and Human Nutrition
dc.subject.keywords Mechanical Engineering
dc.subject.keywords Resistant starch
dc.subject.keywords Fecal analysis
dc.subject.keywords Fourier transform infrared photoacoustic spectroscopy
dc.subject.keywords Partial least squares
dc.subject.keywords Principal component analysis
dc.title Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a56e515d-7fd9-4768-9f73-42a248e128b7
relation.isAuthorOfPublication a9092829-7cfb-4954-8b08-0c0fcaa47f08
relation.isAuthorOfPublication 72e25a81-2813-4101-9504-8f38266732b7
relation.isOrgUnitOfPublication 25913818-6714-4be5-89a6-f70c8facdf7e
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