Evaluation of the Efficacy of an S-INDEL PEDV Strain Administered to Pregnant Gilts against a Virulent Non-S-INDEL PEDV Challenge in Newborn Piglets
Date
2022-08-17
Authors
Schumacher, Loni
Chen, Qi
Fredericks, Lindsay
Gauger, Phillip
Bandrick, Meggan
Keith, Marcia
Magstadt, Drew
Yim-im, Wannarat
Welch, Michael
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MDPI
Abstract
A safe and efficacious live-attenuated vaccine for porcine epidemic diarrhea virus (PEDV) is not commercially available in the United States yet. Two major PEDV strains are currently circulating in US swine: highly virulent non-S-INDEL strain and milder virulent S-INDEL strain. In this study, the safety and protective efficacy of a plaque-purified S-INDEL PEDV isolate formulated as a vaccine candidate was evaluated. Ten pregnant gilts were divided into three groups and orally inoculated at 79 days of gestation and then boosted at 100 days gestation (T01: n = 4, vaccination/challenge; T02: n = 4, non-vaccination/challenge; T03: n = 2, non-vaccination/non-challenge). None of the gilts had adverse clinical signs after vaccination. Only one T01 gilt (#5026) had viral replication and detectible viral RNA in feces. The same gilt had consistent levels of PEDV-specific IgG and IgA antibodies in serum and colostrum/milk. Farrowed piglets at 3 to 5 days of age from T01 and T02 gilts were orally challenged with 103 TCID50/pig of the virulent non-S-INDEL PEDV while T03 piglets were orally inoculated with virus-negative medium. T01 litters had overall lower mortality than T02 (T01 36.4% vs. T02 74.4%). Specifically, there was 0% litter mortality from T01 gilt 5026. Overall, it appears that vaccination of pregnant gilts with S-INDEL PEDV can passively protect piglets if there is virus replication and immune response induction in the pregnant gilts.
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This article is published as Schumacher, Loni, Qi Chen, Lindsay Fredericks, Phillip Gauger, Meggan Bandrick, Marcia Keith, Luis Giménez-Lirola, Drew Magstadt, Wannarat Yim-im, Michael Welch, and Jianqiang Zhang. 2022. "Evaluation of the Efficacy of an S-INDEL PEDV Strain Administered to Pregnant Gilts against a Virulent Non-S-INDEL PEDV Challenge in Newborn Piglets" Viruses 14, no. 8: 1801.
DOI: 10.3390/v14081801.
Copyright 2022 by the authors.
Attribution 4.0 International (CC BY 4.0).
Posted with permission.